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Literature summary extracted from

  • Iwabuchi, T.; Harayama, S.
    Biochemical and genetic characterization of trans-2'-carboxybenzalpyruvate hydratase-aldolase from a phenanthrene-degrading Nocardioides strain (1998), J. Bacteriol., 180, 945-949.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.34 expression in Escherichia coli Nocardioides sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.34 0.05
-
 (E)-2'-carboxybenzylidenepyruvate pH 7.0, 25°C Nocardioides sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.34 35000
-
 3 * 35000, calculated from sequence Nocardioides sp.
4.1.2.34 38000
-
 3 * 38000, SDS-PAGE Nocardioides sp.
4.1.2.34 113000
-
 gel filtration Nocardioides sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O Nocardioides sp. the enzyme is involved in the degradation of phenanthrene 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O Nocardioides sp. KP7 the enzyme is involved in the degradation of phenanthrene 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.34 Nocardioides sp. Q79EM8
-
-
4.1.2.34 Nocardioides sp. KP7 Q79EM8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.34
-
 Nocardioides sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.34 culture condition:phenanthrene-grown cell
-
 Nocardioides sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.34 11.5
-
-
 Nocardioides sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O the enzyme is involved in the degradation of phenanthrene Nocardioides sp. 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298 Nocardioides sp. 2-carboxybenzaldehyde + pyruvate i.e. 2-carboxybenzaldehyde + pyruvate r
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O the enzyme is involved in the degradation of phenanthrene Nocardioides sp. KP7 2-carboxybenzaldehyde + pyruvate i.e.2-carboxybenzaldehyde + pyruvate r
4.1.2.34 (3E)-4-(2-carboxyphenyl)-2-oxobut-3-enoate + H2O i.e. 2'-carboxybenzalpyruvate, equilibrium constant is 298 Nocardioides sp. KP7 2-carboxybenzaldehyde + pyruvate i.e. 2-carboxybenzaldehyde + pyruvate r
4.1.2.34 additional information no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate Nocardioides sp. ?
-
 ?
4.1.2.34 additional information no activity with trans-2'-hydroxycinnamate, benzalpyruvate, and trans-2'-methoxybenzalpyruvate Nocardioides sp. KP7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.34 trimer 3 * 38000, SDS-PAGE Nocardioides sp.
4.1.2.34 trimer 3 * 35000, calculated from sequence Nocardioides sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.2.34 phdJ
-
 Nocardioides sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.34 45
-
-
 Nocardioides sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.34 13
-
 (E)-2'-carboxybenzylidenepyruvate pH 7.0, 25°C Nocardioides sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.34 7.5
-
-
 Nocardioides sp.