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Literature summary extracted from
- Purification and characterization of D-2-haloacid dehalogenase from Pseudomonas putida strain AJ1/23 (1990), J. Gen. Microbiol., 136, 881-886.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.8.1.9 | Cu2+ | - |
Pseudomonas putida |  |
| 3.8.1.9 | thiol reagents | not | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.9 | 1.99 | - |
DL-2-bromopropionate | pH 7.5, D-2-haloacid dehalogenase subtype | Pseudomonas putida | |
| 3.8.1.9 | 27.5 | - |
Monochloroacetate | pH 7.5, D-2-haloacid dehalogenase subtype | Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.9 | 135000 | - |
- |
Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.9 | (R)-2-haloacid + H2O | Pseudomonas putida | - |
(S)-2-hydroxyacid + halide | - |
? | |
| 3.8.1.9 | (R)-2-haloacid + H2O | Pseudomonas putida AJ1/23 | - |
(S)-2-hydroxyacid + halide | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.9 | Pseudomonas putida | - |
- |
- |
| 3.8.1.9 | Pseudomonas putida AJ1/23 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.9 | (R)-2-haloacid + H2O | - |
Pseudomonas putida | (S)-2-hydroxyacid + halide | - |
? | |
| 3.8.1.9 | (R)-2-haloacid + H2O | - |
Pseudomonas putida AJ1/23 | (S)-2-hydroxyacid + halide | - |
? | |
| 3.8.1.9 | DL-2-bromopropionate + H2O | - |
Pseudomonas putida | lactate + HBr | - |
? | |
| 3.8.1.9 | DL-2-bromopropionate + H2O | - |
Pseudomonas putida AJ1/23 | lactate + HBr | - |
? | |
| 3.8.1.9 | DL-2-chloropropionate + H2O | - |
Pseudomonas putida | lactate + HCl | - |
? | |
| 3.8.1.9 | DL-2-chloropropionate + H2O | - |
Pseudomonas putida AJ1/23 | lactate + HCl | - |
? | |
| 3.8.1.9 | monobromoacetate + H2O | - |
Pseudomonas putida | glycolate + HBr | - |
? | |
| 3.8.1.9 | monobromoacetate + H2O | - |
Pseudomonas putida AJ1/23 | glycolate + HBr | - |
? | |
| 3.8.1.9 | monochloroacetate + H2O | - |
Pseudomonas putida | glycolate + HCl | - |
? | |
| 3.8.1.9 | monochloroacetate + H2O | - |
Pseudomonas putida AJ1/23 | glycolate + HCl | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.8.1.9 | tetramer | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.9 | 50 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.9 | 40 | - |
strain AJ1/23, D-2-haloacid dehalogenase subtype, activity loss above 40°C, rapid protein denaturation and precipitation at 56°C | Pseudomonas putida |
| 3.8.1.9 | 50 | - |
12 min, about 90% loss of activity | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.9 | 9.5 | - |
- |
Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.8.1.9 | 8 | 10 | - |
Pseudomonas putida |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.8.1.9 | Pseudomonas putida | chromatofocusing | - |
5 |
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