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Literature summary extracted from

  • Cairns, S.S.; Cornish, A.; Cooper, R.A.
    Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkonoate dehalogenases of opposite stereospecificity (1996), Eur. J. Biochem., 235, 744-749.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expression in Escherichia coli Rhizobium sp.
3.8.1.9 expression in Escherichia coli Rhizobium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 30868
-
 2 * 31000 SDS-PAGE, 2 * 30868, calculated from amino acid sequence Rhizobium sp.
3.8.1.2 31000
-
 2 * 31000 SDS-PAGE, 2 * 30868, calculated from amino acid sequence Rhizobium sp.
3.8.1.2 60000
-
 gel filtration Rhizobium sp.
3.8.1.9 29000
-
 2 * 29000, SDS-PAGE, 2 * 29383, calculated from amino acid sequence Rhizobium sp.
3.8.1.9 29383
-
 2 * 29000, SDS-PAGE, 2 * 29383, calculated from amino acid sequence Rhizobium sp.
3.8.1.9 60000
-
 gel filtration Rhizobium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Rhizobium sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Rhizobium sp.
-
-
-
3.8.1.9 Rhizobium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Rhizobium sp.
3.8.1.9
-
 Rhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Rhizobium sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 dichloroacetic acid + H2O
-
 Rhizobium sp. ?
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Rhizobium sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O
-
 Rhizobium sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 monochloroacetate + H2O
-
 Rhizobium sp. glycolate + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 dimer 2 * 31000 SDS-PAGE, 2 * 30868, calculated from amino acid sequence Rhizobium sp.
3.8.1.9 dimer 2 * 29000, SDS-PAGE, 2 * 29383, calculated from amino acid sequence Rhizobium sp.