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Literature summary extracted from

  • Minami, R.; Uchiyama, K.; Murakami, T.; Kawai, J.; Mikami, K.; Yamada, T.; Yokoi, D.; Ito, H.; Matsui, H.; Honma, M.
    Properties, sequence, and synthesis in Escherichia coli of 1-aminocyclopropane-1-carboxylate deaminase from Hansenula saturnus (1998), J. Biochem., 123, 1112-1118.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.7 subcloned into pET-11d and expressed in Escherichia coli Cyberlindnera saturnus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.7 D-Ala
-
 Cyberlindnera saturnus
3.5.99.7 D-Ser
-
 Cyberlindnera saturnus
3.5.99.7 homoserine
-
 Cyberlindnera saturnus
3.5.99.7 L-2-aminobutanoate
-
 Cyberlindnera saturnus
3.5.99.7 L-Ala
-
 Cyberlindnera saturnus
3.5.99.7 L-Ser
-
 Cyberlindnera saturnus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.7 2.6
-
 1-aminocyclopropane-1-carboxylate
-
 Cyberlindnera saturnus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.7 40000
-
 2 * 40000, SDS-PAGE Cyberlindnera saturnus
3.5.99.7 69000
-
 gel filtration, disc gel electrophoresis Cyberlindnera saturnus

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.7 Cyberlindnera saturnus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.7
-
 Cyberlindnera saturnus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.99.7 additional information
-
-
 Cyberlindnera saturnus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O
-
 Cyberlindnera saturnus 2-oxobutanoate + NH3
-
 ?
3.5.99.7 coronamic acid + H2O DL-coronamic acid Cyberlindnera saturnus 2-oxobutanoate + NH3
-
 ?
3.5.99.7 D-Ser + H2O
-
 Cyberlindnera saturnus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.7 dimer 2 * 40000, SDS-PAGE Cyberlindnera saturnus