Literature summary extracted from

Montero-Moran, G.M.; Horjales, E.; Calcagno, M.L.; Altamirano, M.M.
Tyr254 hydroxyl group acts as a two-way switch mechanism in the coupling of heterotrophic and homotropic effects in Escherichia coli glucosamine-6-phosphate deaminase (1998), Biochemistry, 37, 7844-7849.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.5.99.6	N-acetylglucosamine 6-phosphate	-	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.5.99.6	Y254F	the mutant is less active than wild-type enzyme, the replacement causes an uncoupling of the homotropic and heterotrophic effects	Escherichia coli
3.5.99.6	Y254T	the mutation results in pure K-system with a similar catalytic activity to that of the wild-type enzyme, mutant displays kcat values similar to the wild-type enzyme	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.99.6	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.99.6	wild-type enzyme and two mutants	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.99.6	D-glucosamine 6-phosphate + H2O	-	Escherichia coli	D-fructose 6-phosphate + NH3	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.99.6	hexamer	enzyme is a dimer of trimers	Escherichia coli

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.5.99.6	11.4	-	D-glucosamine 6-phosphate	pH 7.7, 30°C, mutant enzyme Y254F	Escherichia coli
3.5.99.6	75	-	D-glucosamine 6-phosphate	pH 7.7, 30°C, mutant enzyme Y254F in presence of N-acetylglucosamine 6-phosphate	Escherichia coli
3.5.99.6	218	-	D-glucosamine 6-phosphate	pH 7.7, 30°C, mutant enzyme Y254T	Escherichia coli
3.5.99.6	248	-	D-glucosamine 6-phosphate	pH 7.7, 30°C, mutant enzyme Y254T in presence of N-acetylglucosamine 6-phosphate	Escherichia coli
3.5.99.6	292	-	D-glucosamine 6-phosphate	pH 7.7, 30°C, wild-type enzyme	Escherichia coli
3.5.99.6	320	-	D-glucosamine 6-phosphate	pH 7.7, 30°C, wild-type enzyme in presence of N-acetylglucosamine 6-phosphate	Escherichia coli

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Release 2023.1 (January 2023)