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Literature summary extracted from

  • Fromant, M.; Ferri-Fioni, M.L.; Plateau, P.; Blanquet, S.
    Peptidyl-tRNA hydrolase from Sulfolobus solfataricus (2003), Nucleic Acids Res., 31, 3227-3235.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.29 spermidine 0.1 mM spermidine HCl, 73fold activation Saccharolobus solfataricus
3.1.1.29 spermidine-HCl 0.1 mM, 73fold activation Saccharolobus solfataricus
3.1.1.29 spermidine-HCl activates about 80fold, optimally at 0.1 mM Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 expression in Escherichia coli Saccharolobus solfataricus
3.1.1.29 gene SS00175, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deficient Escherichia coli mutant strain, and of two Saccharomyces cerevisiae gene YHR189w or YBL057c disruption mutants, overview, expression of PTH in Escherichia coli strain XL 1-Blue Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.29 non-esterified tRNALys
-
 Saccharolobus solfataricus
3.1.1.29 tRNA unfractionated Escherichia coli tRNA; unfractionated from Escherichia coli Saccharolobus solfataricus
3.1.1.29 tRNA-formyl-methionine from Escherichia coli Saccharolobus solfataricus
3.1.1.29 tRNA-lysine from Escherichia coli Saccharolobus solfataricus
3.1.1.29 tRNAfMet tRNAfMet from Escherichia coli Saccharolobus solfataricus
3.1.1.29 tRNALys tRNALys from Escherichia coli Saccharolobus solfataricus
3.1.1.29 Uncharged tRNA e.g. from Escherichia coli, at concentrations of 0.01 mM or above Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.29 0.0000028
-
 diacetyl-Lys-tRNALys pH 7.5, 50°C, dephosphorylated diacetyl-Lys-tRNALys Saccharolobus solfataricus
3.1.1.29 0.0000028
-
 diacetyl-lysyl-tRNALys dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 0.000003
-
 dephosphorylated diacyl-lysine-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.000011
-
 diacetyl-Lys-tRNALys pH 7.5, 50°C Saccharolobus solfataricus
3.1.1.29 0.000011
-
 diacetyl-lysine-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.000011
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 0.000012
-
 formyl-Met-tRNAfMet pH 7.5, 50°C Saccharolobus solfataricus
3.1.1.29 0.000012
-
 formyl-methionyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.000012
-
 formyl-methionyl-tRNAfMet pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 0.000016
-
 acetyl-His-tRNAHis pH 7.5, 50°C Saccharolobus solfataricus
3.1.1.29 0.000016
-
 acetyl-histidyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.000016
-
 acetyl-histidyl-tRNAHis pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 0.00003
-
 dephosphorylated formyl-methioninyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.00003
-
 dephosphorylated formyl-Met-tRNAfMet pH 7.5, 50°C, dephosphorylated formyl-Met-tRNAfMet Saccharolobus solfataricus
3.1.1.29 0.00003
-
 formyl-methionyl-tRNAfMet dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.29 K+ 0.3 mM, 77fold activation Saccharolobus solfataricus
3.1.1.29 KCl 300 mM, 77fold activation Saccharolobus solfataricus
3.1.1.29 KCl activates about 80fold, optimally at 300 mM Saccharolobus solfataricus
3.1.1.29 Mg2+ 40 mM MgCl2, about 88fold activation Saccharolobus solfataricus
3.1.1.29 Mg2+ activates about 80fold, optimally at 40 mM Saccharolobus solfataricus
3.1.1.29 MgCl2 40 mM, approx. 88fold activation Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.29 13100
-
 2 * 13100, deduced from nucleotide sequence Saccharolobus solfataricus
3.1.1.29 15000
-
 x * 15000, SDS-PAGE Saccharolobus solfataricus
3.1.1.29 15000
-
 2 * 15000, recombinant enzyme, SDS-PAGE Saccharolobus solfataricus
3.1.1.29 25000
-
 gel filtration Saccharolobus solfataricus
3.1.1.29 25000
-
 recombinant enzyme, gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.29 diacetyl-lysyl-tRNALys + H2O Saccharolobus solfataricus
-
 diacetyl-lysine + tRNALys
-
 ?
3.1.1.29 N-substituted aminoacyl-tRNA + H2O Escherichia coli
-
 N-substituted amino acid + tRNA
-
 ?
3.1.1.29 N-substituted aminoacyl-tRNA + H2O Saccharolobus solfataricus
-
 N-substituted amino acid + tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Escherichia coli
-
-
-
3.1.1.29 Saccharolobus solfataricus
-
-
-
3.1.1.29 Saccharolobus solfataricus
-
 gene SSO0175
-
3.1.1.29 Saccharomyces cerevisiae
-
 YHR189w and YBL057c gene products
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29
-
 Escherichia coli
3.1.1.29
-
 Saccharolobus solfataricus
3.1.1.29 recombinant PTH, heat, ammonium sulfate, Superdex 75, HI-Propyl, SP-Sepharose Saccharolobus solfataricus
3.1.1.29 recomninant PTH from Escherichia coli strain XL 1-Blue 8500fold by ultracentrifugation, heat treatment, ammonium sulfate fractionation, gel filtration, hydrophobic interaction chromatography, and ion exchange chromatography to homogeneity Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.29 additional information
-
-
 Saccharolobus solfataricus
3.1.1.29 0.078
-
 substrate D-tyrosyl-tRNA, 37°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.15
-
 substrae D-tyrosyl-tRNA Escherichia coli
3.1.1.29 4.14
-
-
 Saccharolobus solfataricus
3.1.1.29 4.14
-
 purified recombinant enzyme Saccharolobus solfataricus
3.1.1.29 30
-
 substrate diacetyl-lysine-tRNA Escherichia coli
3.1.1.29 31.8
-
 substrate diacetyl-lysine tRNA, 37°C, pH 7.5 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 5'-dephosphorylated N,N-diacetyl-L-lysyl-tRNALys + H2O
-
 Saccharolobus solfataricus N,N-diacetyl-L-lysine + 5'-dephosphorylated tRNALys
-
 ?
3.1.1.29 5'-dephosphorylated N-formyl-L-methionyl-tRNA + H2O
-
 Saccharolobus solfataricus N-formyl-L-methionine + 5'-dephosphorylated tRNA
-
 ?
3.1.1.29 acetyl-histidine-tRNA + H2O
-
 Saccharolobus solfataricus acetyl-histidine + tRNA
-
 ?
3.1.1.29 acetyl-histidyl-tRNAHis + H2O
-
 Saccharolobus solfataricus acetyl-histidine + tRNAHis
-
 ?
3.1.1.29 D-tyrosine-tRNA + H2O
-
 Escherichia coli D-tyrosine + tRNA
-
 ?
3.1.1.29 D-tyrosine-tRNA + H2O
-
 Saccharolobus solfataricus D-tyrosine + tRNA
-
 ?
3.1.1.29 dephosphorylated diacyl-lysine-tRNA + H2O
-
 Saccharolobus solfataricus diacyl-lysine + dephosphorylated tRNA
-
 ?
3.1.1.29 dephosphorylated formyl-Met-tRNAfMet + H2O
-
 Saccharolobus solfataricus formyl-Met + dephosphorylated tRNAfMet
-
 ?
3.1.1.29 dephosphorylated formyl-methioninyl-tRNA + H2O
-
 Saccharolobus solfataricus formyl-methionine + dephosphorylated tRNA
-
 ?
3.1.1.29 diacetyl-Lys-tRNALys + H2O
-
 Saccharomyces cerevisiae diacetyl-Lys + tRNA
-
 ?
3.1.1.29 diacetyl-Lys-tRNALys + H2O diacetyl-Lys-tRNALys from E. coli Saccharolobus solfataricus diacetyl-Lys + tRNA
-
 ?
3.1.1.29 diacetyl-lysine-tRNA + H2O
-
 Escherichia coli diacetyl-lysine + tRNA
-
 ?
3.1.1.29 diacetyl-lysine-tRNA + H2O
-
 Saccharolobus solfataricus diacetyl-lysine + tRNA
-
 ?
3.1.1.29 diacetyl-lysyl-tRNALys + H2O
-
 Saccharolobus solfataricus diacetyl-lysine + tRNALys
-
 ?
3.1.1.29 diacetyl-lysyl-tRNALys + H2O Escherichia coli diacetyl-lysyl-tRNALys, phosphorylated and dephosphorylated substrate Saccharolobus solfataricus diacetyl-lysine + tRNALys
-
 ?
3.1.1.29 formyl-Met-tRNAfMet + H2O
-
 Saccharolobus solfataricus formyl-Met + tRNAfMet
-
 ?
3.1.1.29 formyl-methionine-tRNA + H2O
-
 Saccharolobus solfataricus formyl-methionine + tRNA
-
 ?
3.1.1.29 formyl-methioninyl-tRNA + H2O
-
 Saccharolobus solfataricus formyl-methionine + tRNA
-
 ?
3.1.1.29 formyl-methionyl-tRNAfMet + H2O Escherichia coli formyl-methionyltRNAfMet, phosphorylated and dephosphorylated substrate Saccharolobus solfataricus formyl-methionine + tRNAfMet
-
 ?
3.1.1.29 additional information the enzyme is involved in the recycling of peptidyl-tRNA, it shows poor D-aminoacyl-tRNA hydrolysis Saccharolobus solfataricus ?
-
 ?
3.1.1.29 N-acetyl-His-tRNA + H2O
-
 Saccharolobus solfataricus N-acetyl-His + tRNA
-
 ?
3.1.1.29 N-substituted aminoacyl-tRNA + H2O
-
 Escherichia coli N-substituted amino acid + tRNA
-
 ?
3.1.1.29 N-substituted aminoacyl-tRNA + H2O
-
 Saccharolobus solfataricus N-substituted amino acid + tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.29 ? x * 15000, SDS-PAGE Saccharolobus solfataricus
3.1.1.29 dimer 2 * 13100, deduced from nucleotide sequence Saccharolobus solfataricus
3.1.1.29 dimer 2 * 15000, recombinant enzyme, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 peptidyl-tRNA hydrolase
-
 Saccharolobus solfataricus
3.1.1.29 PTH
-
 Escherichia coli
3.1.1.29 PTH
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.29 50
-
 assay at Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.29 0.86
-
 diacetyl-Lys-tRNALys pH 7.5, 50°C, dephosphorylated diacetyl-Lys-tRNALys Saccharolobus solfataricus
3.1.1.29 0.86
-
 dephosphorylated diacyl-lysine-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.86
-
 diacetyl-lysyl-tRNALys dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 1.8
-
 diacetyl-Lys-tRNALys pH 7.5, 50°C Saccharolobus solfataricus
3.1.1.29 1.8
-
 diacetyl-lysine-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 1.8
-
 diacetyl-lysyl-tRNALys pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 3
-
 formyl-Met-tRNAfMet pH 7.5, 50°C Saccharolobus solfataricus
3.1.1.29 3
-
 formyl-methionyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 3
-
 dephosphorylated formyl-methioninyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 3
-
 formyl-methioninyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 3
-
 formyl-methionyl-tRNAfMet phosphorylated and dephosphorylated substrate, pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 3.4
-
 acetyl-His-tRNA pH 7.5, 50°C Saccharolobus solfataricus
3.1.1.29 3.4
-
 acetyl-histidyl-tRNA 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 3.4
-
 acetyl-histidyl-tRNAHis pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 6.08
-
 dephosphorylated diacyl-lysine-tRNA 50°C, pH 7.5 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.29 7.5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.29 0.00005
-
 tRNALys pH 7.5, 50°C, tRNALys from Escherichia coli Saccharolobus solfataricus
3.1.1.29 0.00005
-
 tRNA-lysine tRNA-lysine from Escherichia coli, 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.00005
-
 non-esterified tRNALys pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 0.000095
-
 tRNA pH 7.5, 50°C, unfractionated Escherichia coli tRNA Saccharolobus solfataricus
3.1.1.29 0.000095
-
 tRNA unfractionated tRNA from Escherichia coli, 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.0001
-
 tRNA-formyl-methionine tRNA-ormyl-methionine from Escherichia coli, 50°C, pH 7.5 Saccharolobus solfataricus
3.1.1.29 0.0001
-
 Uncharged tRNA pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus
3.1.1.29 0.00011
-
 tRNAformylMet pH 7.5, 50°C, tRNAformylMet from Escherichia coli Saccharolobus solfataricus
3.1.1.29 0.00011
-
 tRNAfMet pH 7.5, 50°C, recombinant enzyme Saccharolobus solfataricus