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Literature summary extracted from

  • Kirschke, H.; Wiederanders, B.; Brömme, D.; Rinne, A.
    Cathepsin S from bovine spleen. Purification, distribution, intracellular localization and action on proteins (1989), Biochem. J., 264, 467-473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.27
-
 Bos taurus

General Stability

EC Number General Stability Organism
3.4.22.27 the enzyme is partially destroyed by autolysis of the homogenate at pH 4.2 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.27 cytoplasmic vesicle
-
 Bos taurus 31410
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.27 20000
-
 gel filtration Bos taurus
3.4.22.27 23700
-
 x * 23700, calculation from nucleotide sequence Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.27 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.27 glycoprotein the enzyme is expected to contain carbohydrate Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.27
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.27 kidney concentrated to the cells of the proximal tubule Bos taurus
-
3.4.22.27 lung
-
 Bos taurus
-
3.4.22.27 lymph node
-
 Bos taurus
-
3.4.22.27 spleen
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.27 0.582
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.22.27 -10°C, 0.012-0.04 mM cathepsin S, 100 mM sodium acetate buffer, pH 5.5, 1 mM EDTA, stable for 8 months. In presence of 0.5 mM Hg2+ and 1 mM EDTA rapid decrease of about 50% of the activity which then remains unchanged for 3 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.27 Albumin + H2O
-
 Bos taurus ?
-
 ?
3.4.22.27 azocasein + H2O high activity in presence of urea at pH 5.5 Bos taurus ?
-
 ?
3.4.22.27 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Bos taurus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.27 Collagen + H2O
-
 Bos taurus ?
-
 ?
3.4.22.27 haemoglobin + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.27 ? x * 23700, calculation from nucleotide sequence Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.27 40
-
 pH 5.5-7.0, in presence for dithiothreitol and EDTA stable for 240 min. 10-12% of the enzyme activity is lost after 60 min at pH 7.5 or pH 4.0. In absence of dithiothreitol and EDTA the enzyme is inactivated at pH 4.0 by 70% after 60 min Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.27 4
-
 10-12% loss of activity after 60 min in presence of dithiothreitol and EDTA, 70% inactivation after 60 min in absence of dithiothreitol and EDTA Bos taurus
3.4.22.27 5.5 7 40°C, stable for 240 min in presence of dithiothreitol and EDTA Bos taurus
3.4.22.27 7.5
-
 40°C, 10-12% loss of activity after 60 min in presence of dithiothreitol and EDTA Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.27 Bos taurus isoelectric focusing
-
 7