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Literature summary extracted from

  • Ramachandran, R.; Hartmann, C.; Song, H.K.; Huber, R.; Bochtler, M.
    Functional interactions of HslV (ClpQ) with the ATPase HslU (ClpY) (2002), Proc. Natl. Acad. Sci. USA, 99, 7396-7401.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.25.2 HslU protease HslV is activated by the ATPase HslU. Mutations in hslV that disrupt the interaction with the C termini of HslU invariably lead to inactive enzyme Haemophilus influenzae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.25.2 DELTA83-92 hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme Haemophilus influenzae
3.4.25.2 DELTA86-91 hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is unchanged Haemophilus influenzae
3.4.25.2 K28A hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme Haemophilus influenzae
3.4.25.2 R35A hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme Haemophilus influenzae
3.4.25.2 R86D hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme Haemophilus influenzae
3.4.25.2 R89A/K90A hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme Haemophilus influenzae
3.4.25.2 R89D hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is 40-60% of the activity of the wild-type enzyme Haemophilus influenzae
3.4.25.2 R89D/K90E hydrolysis of casein and SulA-MBP is less than 20% of the activity of the wild-type enzyme, hydrolysis of benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is higher than that of the wild-type enzyme Haemophilus influenzae
3.4.25.2 V112A hydrolysis of casein, SulA-MBP or benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin is less than 20% of the activity of the wild-type enzyme Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
3.4.25.2 Haemophilus influenzae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.25.2 benzyloxycarbonyl-GGL-7-amido-4-methylcoumarin + H2O
-
 Haemophilus influenzae benzyloxycarbonyl-GGL + 7-amino-4-methylcoumarin
-
 ?
3.4.25.2 casein + H2O
-
 Haemophilus influenzae ?
-
 ?