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Literature summary extracted from

  • Malm, J.; Hellman, J.; Hogg, P.; Lilja, H.
    Enzymatic action of prostate-specific antigen (PSA or hK3): substrate specificity and regulation by Zn(2+), a tight-binding inhibitor (2000), Prostate, 45, 132-139.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.77 stable at 0.5 M urea in the activity assay, which is needed to prevent coagualtion of th semenogelins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.77 alpha1-Aantichymotrypsin complex formation Homo sapiens
3.4.21.77 Cd2+ competitive to other metal ions Homo sapiens
3.4.21.77 Co2+ competitive to other metal ions Homo sapiens
3.4.21.77 Cu2+ competitive to other metal ions Homo sapiens
3.4.21.77 Hg2+ competitive to other metal ions Homo sapiens
3.4.21.77 Zn2+ 50% inhibition at 0.02 mM, 25fold molar excess of Zn2+ to enzyme; competitive to substrate and other metal ions, modeling; strong, tight-binding inhibitor Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.77 extracellular secretion Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.77 polypeptide + H2O Homo sapiens
-
 peptides
-
 ?
3.4.21.77 semenogelin + H2O Homo sapiens semenogelins I and II, cleavage results in liquefaction and release of motil spermatozoa semenogelin fractions
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.77 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.77 Preferential cleavage: -Tyr-/- cleaves at the C-terminal side of several amino acids residues, like tyrosine, glutamine, histidine, asparic acid, leucine, serine, and asparagine, not at arginine, lysine, phenylalanine, tryptophan, or methionine residues Homo sapiens
a
3.4.21.77 Preferential cleavage: -Tyr-/- enzyme shows a substrate specificity different from trypsin, chymotrypsin, and kallikrein 1 and 2 Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.77 semen
-
 Homo sapiens
-
3.4.21.77 seminal plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.77 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O chromogenic substrate Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
 ?
3.4.21.77 methoxy-succinyl-Arg-Pro-Tyr-4-nitroanilide + H2O i.e. S2586 Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline
-
 ?
3.4.21.77 polypeptide + H2O
-
 Homo sapiens peptides
-
 ?
3.4.21.77 semenogelin + H2O semenogelins I and II Homo sapiens semenogelin fragments
-
 ?
3.4.21.77 semenogelin + H2O semenogelin I contains 18 cleavage sites, semenogelin II contains 16 cleavage sites Homo sapiens semenogelin fragments
-
 ?
3.4.21.77 semenogelin + H2O semenogelins I and II, cleavage results in liquefaction and release of motil spermatozoa Homo sapiens semenogelin fractions
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.77 K3
-
 Homo sapiens
3.4.21.77 prostate-specific antigen
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.77 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.77 8
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.77 0.0056
-
 Zn2+ pH 8.0, 37°C, with methoxy-succinyl-Arg-Pro-Tyr-4-nitrophenyl Homo sapiens