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Literature summary extracted from

  • Fiedler, F.
    Effects of secondary interactions on the kinetics of peptide and peptide ester hydrolysis by tissue kallikrein trypsin (1987), Eur. J. Biochem., 163, 303-312.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.35 0.0114
-
 benzyloxycarbonyl-Pro-Phe-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.0244
-
 acetyl-Phe-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.0249
-
 acetyl-Leu-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.027
-
 acetyl-Trp-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.04
-
 acetyl-Phe-Arg-Ser-Val-NH2 pH 9.0, 25°C Sus scrofa
3.4.21.35 0.057
-
 PFRSVQ pH 9.0, 25°C Sus scrofa
3.4.21.35 0.064
-
 FSPFRSVQ pH 9.0, 25°C Sus scrofa
3.4.21.35 0.066
-
 RPPGFSPFRSVQ pH 9.0, 25°C Sus scrofa
3.4.21.35 0.08
-
 acetyl-2-aminohexanoic acid-Arg methyl ester
-
 Sus scrofa
3.4.21.35 0.132
-
 acetyl-Tyr-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.153
-
 acetyl-Pro-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.182
-
 acetyl-Met-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.199
-
 acetyl-Ile-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.209
-
 benzyloxycarbonyl-Pro-Gly-Arg methyl ester
-
 Sus scrofa
3.4.21.35 0.21
-
 acetyl-Phe-Arg-Ser-NH2 pH 9.0, 25°C Sus scrofa
3.4.21.35 0.283
-
 acetyl-Val-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.31
-
 acetyl-Phe-Arg-Ser-Val-Gln pH 9.0, 25°C Sus scrofa
3.4.21.35 0.6
-
 acetyl-Ala-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.77
-
 acetyl-Gly-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 0.95
-
 acetyl-D-Phe-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 2.2
-
 Phe-Arg-Ser-Val-Gln pH 9.0, 25°C Sus scrofa
3.4.21.35 2.5
-
 acetyl-D-Ala-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 26
-
 acetyl-Gly-Arg-Ser-Val-Gln pH 9.0, 25°C Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.35 polypeptide + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.35 Sus scrofa
-
 form B
-
3.4.21.35 Sus scrofa
-
 beta-kallikrein
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.35 acetyl-2-aminohexanoic acid-Arg methyl ester + H2O
-
 Sus scrofa acetyl-2-aminohexanoic acid-Arg + methanol
-
 ?
3.4.21.35 acetyl-Ala-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Ala-Arg + methanol
-
 ?
3.4.21.35 acetyl-D-Ala-Arg methyl ester + H2O
-
 Sus scrofa acetyl-D-Ala-Arg + methanol
-
 ?
3.4.21.35 acetyl-D-Phe-Arg methyl ester + H2O
-
 Sus scrofa acetyl-D-Phe-Arg + methanol
-
 ?
3.4.21.35 acetyl-Gly-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Gly-Arg + methanol
-
 ?
3.4.21.35 acetyl-Gly-Arg-Ser-Val-Gln + H2O
-
 Sus scrofa acetyl-Gly-Arg + Ser-Val-Gln
-
 ?
3.4.21.35 acetyl-Ile-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Ile-Arg + methanol
-
 ?
3.4.21.35 acetyl-Leu-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Leu-Arg + methanol
-
 ?
3.4.21.35 acetyl-Met-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Met-Arg + methanol
-
 ?
3.4.21.35 acetyl-Phe-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Phe-Arg + methanol
-
 ?
3.4.21.35 acetyl-Phe-Arg-Ser-NH2 + H2O
-
 Sus scrofa acetyl-Phe-Arg + Ser-NH2
-
 ?
3.4.21.35 acetyl-Phe-Arg-Ser-Val-Gln + H2O
-
 Sus scrofa acetyl-Phe-Arg + Ser-Val-Gln
-
 ?
3.4.21.35 acetyl-Phe-Arg-Ser-Val-NH2 + H2O
-
 Sus scrofa acetyl-Phe-Arg + Ser-Val-NH2
-
 ?
3.4.21.35 acetyl-Pro-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Pro-Arg + methanol
-
 ?
3.4.21.35 acetyl-Trp-2-aminohexanoic acid methyl ester + H2O
-
 Sus scrofa ?
-
 ?
3.4.21.35 acetyl-Trp-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Trp-Arg + methanol
-
 ?
3.4.21.35 acetyl-Tyr-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Tyr-Arg + methanol
-
 ?
3.4.21.35 acetyl-Val-Arg methyl ester + H2O
-
 Sus scrofa acetyl-Val-Arg + methanol
-
 ?
3.4.21.35 benzyloxycarbonyl-Pro-Gly-Arg methyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-Pro-Gly-Arg + methanol
-
 ?
3.4.21.35 benzyloxycarbonyl-Pro-Phe-Arg methyl ester + H2O
-
 Sus scrofa benzyloxycarbonyl-Pro-Phe-Arg + methanol
-
 ?
3.4.21.35 FSPFRSVQ + H2O
-
 Sus scrofa FSPFR + SVQ
-
 ?
3.4.21.35 PFRSVQ + H2O
-
 Sus scrofa PFR + SVQ
-
 ?
3.4.21.35 Phe-Arg-Ser-Val-Gln + H2O
-
 Sus scrofa Phe-Arg + Ser-Val-Gln
-
 ?
3.4.21.35 polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
3.4.21.35 RPPGFSPFRSVQ + H2O
-
 Sus scrofa RPPGFSPFR + SVQ
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.35 25
-
 assay at Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.35 0.77
-
 acetyl-Gly-Arg-Ser-Val-Gln pH 9.0, 25°C Sus scrofa
3.4.21.35 2.1
-
 acetyl-Phe-Arg-Ser-NH2 pH 9.0, 25°C Sus scrofa
3.4.21.35 2.8
-
 Phe-Arg-Ser-Val-Gln pH 9.0, 25°C Sus scrofa
3.4.21.35 4.6
-
 acetyl-D-Ala-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 5.7
-
 PFRSVQ pH 9.0, 25°C Sus scrofa
3.4.21.35 5.7
-
 RPPGFSPFRSVQ pH 9.0, 25°C Sus scrofa
3.4.21.35 6.4
-
 FSPFRSVQ pH 9.0, 25°C Sus scrofa
3.4.21.35 6.8
-
 acetyl-Phe-Arg-Ser-Val-Gln pH 9.0, 25°C Sus scrofa
3.4.21.35 11.6
-
 acetyl-Phe-Arg-Ser-Val-NH2 pH 9.0, 25°C Sus scrofa
3.4.21.35 49
-
 acetyl-D-Phe-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 259
-
 benzyloxycarbonyl-Pro-Gly-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 385
-
 acetyl-Gly-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 438
-
 acetyl-Trp-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 537
-
 benzyloxycarbonyl-Pro-Phe-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 634
-
 acetyl-Leu-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 680
-
 acetyl-Tyr-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 682
-
 acetyl-Ala-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 727
-
 acetyl-Ile-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 806
-
 acetyl-Pro-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 915
-
 acetyl-Phe-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 964
-
 acetyl-Val-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 1180
-
 acetyl-2-aminohexanoic acid-Arg methyl ester pH 9.0, 25°C Sus scrofa
3.4.21.35 1380
-
 acetyl-Met-Arg methyl ester pH 9.0, 25°C Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.35 9
-
 assay at Sus scrofa