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Literature summary extracted from

  • Ryu, O.; Hu, J.C.; Yamakoshi, Y.; Villemain, J.L.; Cao, X.; Zhang, C.; Bartlett, J.D.; Simmer, J.P.
    Porcine kallikrein-4 activation, glycosylation, activity, and expression in prokaryotic and eukaryotic hosts (2002), Eur. J. Oral Sci., 110, 358-365.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.B12 additional information no activation of recombinant zymogen by native KLK4 enzyme in vitro Sus scrofa
3.4.21.B12 recombinant pig enamelysin can activate the recombinant zymogen in vitro Sus scrofa
3.4.21.B12 Thermolysin can activate the recombinant zymogen in vitro Sus scrofa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B12 expression in Escherichia coli strain AD 494 (DE3) with and without the pro-peptide Sus scrofa
3.4.21.B12 expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus Sus scrofa
3.4.21.B12 expression of inactive zymogen in human 293 kidney cells Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B12 cytosol
-
 Sus scrofa 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B12 28000
-
 x * 28000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B12 additional information Sus scrofa enzyme participates in the degradation of enamel proteins in vivo ?
-
 ?
3.4.21.B12 polypeptide + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B12 Sus scrofa Q9XSN6 gene KLK4
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.B12 glycoprotein 2 N-glycosylated forms of different MW: 34 kDa and 37 kDa Sus scrofa
3.4.21.B12 glycoprotein deglycosylation abolishes enzyme activity Sus scrofa
3.4.21.B12 proteolytic modification no activation of recombinant zymogen by native KLK4 enzyme in vitro Sus scrofa
3.4.21.B12 proteolytic modification recombinant zymogen can be activated in vitro by thermolysin and by recombinant pig enamelysin Sus scrofa

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B12 native enzyme from developing teeth Sus scrofa
3.4.21.B12 recombinant enzyme from 293 kidney cells Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.21.B12 proteolysis of polypeptides enzyme is a serine protease Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B12 enamel transition-stage developing matrix Sus scrofa
-
3.4.21.B12 tooth
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B12 amelogenin + H2O recombinant substrate from pig expressed in Escherichia coli strain XL-1 blue as His-tagged protein Sus scrofa amelogenin fragments product identification by N-terminal sequencing and mass spectrometry ?
3.4.21.B12 casein + H2O zymograph assay Sus scrofa ?
-
 ?
3.4.21.B12 Gelatin + H2O zymograph assay Sus scrofa ?
-
 ?
3.4.21.B12 additional information recombinant zymogen can be activated by thermolysin Sus scrofa ?
-
 ?
3.4.21.B12 additional information enzyme participates in the degradation of enamel proteins in vivo Sus scrofa ?
-
 ?
3.4.21.B12 polypeptide + H2O
-
 Sus scrofa peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B12 ? x * 34000-37000, glycosylated enzyme, 2 glycosylation forms, SDS-PAGE Sus scrofa
3.4.21.B12 ? x * 28000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B12 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B12 7.2
-
 assay at Sus scrofa