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Literature summary extracted from

  • Reverter, D.; Garcia-Saez, I.; Catasus, L.; Vendrell, J.; Coll, M.; Aviles, F.X.
    Characterization and preliminary x-ray diffraction analysis of human pancreatic procarboxypeptidase A2 (1997), FEBS Lett., 420, 7-10.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.15 procarboxypeptidase A2 is expressed in Pichia pastoris Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.17.15 hanging drop vapour diffusion method at 4°C and at 20°C, procarboxypeptidase A2 is crystallized using vapour diffusion approach. The crystals belong to the monoclinic system spacegroup P21 and present one procarboxypeptidase A2 molecule per asymmetric unit Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.15 0.125
-
 benzyloxycarbonyl-Gly-Gly-Tyr pH 7.5, 25°C Homo sapiens
3.4.17.15 0.146
-
 benzyloxycarbonyl-Gly-Gly-Trp pH 7.5, 25°C Homo sapiens
3.4.17.15 0.175
-
 Benzyloxycarbonyl-Gly-Tyr pH 7.5, 25°C Homo sapiens
3.4.17.15 0.261
-
 benzyloxycarbonyl-Gly-Trp pH 7.5, 25°C Homo sapiens
3.4.17.15 0.372
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 7.5, 25°C Homo sapiens
3.4.17.15 2.028
-
 benzyloxycarbonyl-Gly-Trp pH 7.5, 25°C Homo sapiens
3.4.17.15 2.27
-
 Benzyloxycarbonyl-Gly-Phe pH 7.5, 25°C Homo sapiens
3.4.17.15 5.3
-
 benzyloxycarbonyl-Gly-Gly-Leu pH 7.5, 25°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.15 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.15 proteolytic modification synthesized as procarboxypeptidase A2 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.15
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.15 pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.15 benzyloxycarbonyl-Gly-Gly-Leu + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 benzyloxycarbonyl-Gly-Gly-Phe + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 benzyloxycarbonyl-Gly-Gly-Trp + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 benzyloxycarbonyl-Gly-Gly-Tyr + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 benzyloxycarbonyl-Gly-Phe + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 benzyloxycarbonyl-Gly-Trp + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 benzyloxycarbonyl-Gly-Tyr + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.15 additional information no measurable activity with benzyloxycarbonyl-Gly-Gly-Val, benzyloxycarbonyl-Gly-Gly-Ala und benzyloxycarbonyl-Gly-Gly-Ser. CPA2 is the only reported carboxypeptidase with specificity for C-terminal Trp residues Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.15 9.7
-
 Benzyloxycarbonyl-Gly-Tyr pH 7.5, 25°C Homo sapiens
3.4.17.15 11.8
-
 benzyloxycarbonyl-Gly-Gly-Leu pH 7.5, 25°C Homo sapiens
3.4.17.15 16.1
-
 Benzyloxycarbonyl-Gly-Phe pH 7.5, 25°C Homo sapiens
3.4.17.15 58.1
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 7.5, 25°C Homo sapiens
3.4.17.15 70
-
 benzyloxycarbonyl-Gly-Gly-Tyr pH 7.5, 25°C Homo sapiens
3.4.17.15 90.3
-
 benzyloxycarbonyl-Gly-Gly-Phe pH 7.5, 25°C Homo sapiens