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Literature summary extracted from
- Human carboxypeptidase M. Purification and characterization of a membrane-bound carboxypeptidase that cleaves peptide hormones (1989), J. Biol. Chem., 264, 2236-2241.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.12 | 1,10-phenanthroline | - |
Homo sapiens |  |
| 3.4.17.12 | 2-mercaptomethyl-3-guanidinoethyl thiopropanoic acid | - |
Homo sapiens | |
| 3.4.17.12 | Cd(CH3COO)2 | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.12 | 0.016 | - |
bradykinin | - |
Homo sapiens | |
| 3.4.17.12 | 0.046 | - |
Met5-Arg6-enkephalin | - |
Homo sapiens | |
| 3.4.17.12 | 0.063 | - |
Leu5-Arg6-enkephalin | - |
Homo sapiens | |
| 3.4.17.12 | 0.375 | - |
Met5-Lys6-enkephalin | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.17.12 | plasma membrane | - |
Homo sapiens | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.12 | Co2+ | CoCl2 activates | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.12 | 62000 | - |
x * 62000, human, SDS-PAGE with or without reduction | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.12 | additional information | Homo sapiens | the enzyme could inactivate or modulate the activity of peptide hormones either before or after their interaction with plasma membrane receptors | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.12 | Homo sapiens | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.17.12 | glycoprotein | carbohydrate content: 23% by weight | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.12 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.12 | placenta | microvilli | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.12 | benzoyl-Ala-Lys + H2O | - |
Homo sapiens | benzoyl-Ala + Lys | - |
? | |
| 3.4.17.12 | benzoyl-Gly-Arg + H2O | - |
Homo sapiens | benzoyl-Gly + Arg | - |
? | |
| 3.4.17.12 | benzoyl-Gly-Lys + H2O | - |
Homo sapiens | benzoyl-Gly + Lys | - |
? | |
| 3.4.17.12 | bradykinin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.17.12 | Dansyl-Ala-Arg + H2O | - |
Homo sapiens | Dansyl-Ala + Arg | - |
? | |
| 3.4.17.12 | dynorphin A(1-13) + H2O | i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys | Homo sapiens | Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu + Lys | - |
? | |
| 3.4.17.12 | Leu5-Arg6-enkephalin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.17.12 | Met5-Arg6-enkephalin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.17.12 | Met5-Lys6-enkephalin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.17.12 | additional information | carboxypeptidase M does not hydrolyze the peptidyl dipeptidase substrate furylacryloyl-Phe-Gly-Gly or the carboxypeptidase A substrates benzoyl-Gly-phenyllactic acid and furylacryloyl-Phe-Phe | Homo sapiens | ? | - |
? | |
| 3.4.17.12 | additional information | the enzyme could inactivate or modulate the activity of peptide hormones either before or after their interaction with plasma membrane receptors | Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.12 | ? | x * 62000, human, SDS-PAGE with or without reduction | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.12 | 1.77 | - |
Leu5-Arg6-enkephalin | - |
Homo sapiens | |
| 3.4.17.12 | 2.45 | - |
bradykinin | - |
Homo sapiens | |
| 3.4.17.12 | 11.1 | - |
Met5-Lys6-enkephalin | - |
Homo sapiens | |
| 3.4.17.12 | 15.6 | - |
Met5-Arg6-enkephalin | - |
Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.12 | 7 | - |
hydrolysis of benzoyl-Ala-Lys | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.12 | 5.5 | 9 | pH 5.5: 25% of maximal activity, pH 9.0: about 60% of maximal activity | Homo sapiens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.12 | 4 | - |
1 h, room temperature, 33% loss of activity | Homo sapiens |
| 3.4.17.12 | 4.3 | - |
1 h, room temperature, 17% loss of activity | Homo sapiens |
| 3.4.17.12 | 4.5 | 5 | 1 h, room temperature, stable | Homo sapiens |
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