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Literature summary extracted from

  • Goldman, P.; Levy, C.C.
    Carboxypeptidase G: purification and properties (1967), Proc. Natl. Acad. Sci. USA, 58, 1299-1306.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
3.4.17.11 activity can increase up to 30% if the enzyme at all stages of purification is stored at -20°C Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.11 Ac-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Ala-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Benzoyl-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Benzyloxycarbonyl-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Benzyloxycarbonyl-Gly-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Glu-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Gly-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 His-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Ile-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Phe-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
3.4.17.11 Tyr-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.11 Zn2+ required Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.11 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.11
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.11 additional information
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
3.4.17.11 -196°C, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.11 folic acid + H2O
-
 Pseudomonas sp. pteroic acid + glutamic acid
-
 ?
3.4.17.11 methotrexate + H2O
-
 Pseudomonas sp. 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate
-
 ?
3.4.17.11 additional information rapid hydrolysis occurs in linkages of the form RCO-Glu and RCO-Gln, whereas those of the form RCO-D-Glu (D-Glu-D-Glu and Z-D-Glu) are not attacked Pseudomonas sp. ?
-
 ?
3.4.17.11 additional information no hydrolysis of glutamate or glutamine lacking a free carboxyl alpha-carboxyl Pseudomonas sp. ?
-
 ?