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Literature summary extracted from
- Kinetic studies of carboxypeptidase Y. I. Kinetic parameters for the hydrolysis of synthetic substrates (1975), J. Biochem., 77, 69-79.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.16.5 | acetyl-D-Phe ethyl ester | - |
Saccharomyces cerevisiae |  |
| 3.4.16.5 | benzyloxycarbonyl-D-Phe | - |
Saccharomyces cerevisiae | |
| 3.4.16.5 | benzyloxycarbonyl-D-Phe-D-Leu | - |
Saccharomyces cerevisiae | |
| 3.4.16.5 | benzyloxycarbonyl-Phe | - |
Saccharomyces cerevisiae | |
| 3.4.16.5 | dimethylformamide | - |
Saccharomyces cerevisiae | |
| 3.4.16.5 | ethanol | - |
Saccharomyces cerevisiae | |
| 3.4.16.5 | L-amino acids | competitive | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.16.5 | 0.053 | - |
benzyloxycarbonyl-Glu-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.068 | - |
benzyloxycarbonyl-Leu-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.1 | - |
Benzyloxycarbonyl-Phe-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.1 | - |
Benzyloxycarbonyl-His-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.1 | - |
benzyloxycarbonyl-Leu-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.13 | - |
benzyloxycarbonyl-Glu-Phe | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.14 | - |
Benzyloxycarbonyl-Glu-Tyr | pH 5.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.23 | - |
Benzyloxycarbonyl-His-Phe | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.36 | - |
benzyloxycarbonyl-Phe-Glu | pH 4.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.38 | - |
Benzyloxycarbonyl-His-Phe | pH 7.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.41 | - |
benzyloxycarbonyl-Phe-Glu | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.45 | - |
benzoyl-Gly-beta-phenyllactate | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.5 | - |
benzyloxycarbonyl-Leu-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.5 | - |
Benzyloxycarbonyl-Glu-Tyr | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.56 | - |
benzyloxycarbonyl-Phe-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.67 | - |
benzyloxycarbonyl-Phe-Pro | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.74 | - |
Acetyl-Phe-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.83 | - |
benzyloxycarbonyl-His-Tyr | pH 5.7, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.9 | - |
benzyloxycarbonyl-Phe-His | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.15 | - |
Benzyloxycarbonyl-Gly-Glu | pH 4.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.2 | - |
acetyl-Phe ethyl ester | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.28 | - |
acetyl-Phe ethyl ester | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.33 | - |
benzyloxycarbonyl-His-Tyr | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.7 | - |
Benzyloxycarbonyl-Gly-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.75 | - |
benzyloxycarbonyl-Gly-Gly | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.82 | - |
benzyloxycarbonyl-His-Tyr | pH 7.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 2.4 | - |
Acetyl-Tyr-ethyl ester | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 3 | - |
Benzyloxycarbonyl-Glu-Tyr | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 3.2 | - |
benzyloxycarbonyl-Phe-His | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 3.26 | - |
Benzyloxycarbonyl-Ala-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 3.3 | - |
benzyloxycarbonyl-Glu-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 4 | - |
benzyloxycarbonyl-Phe-Gly | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 7.7 | - |
benzoyl-Gly-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 9 | - |
benzyloxycarbonyl-Phe-beta-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 9.09 | - |
Benzyloxycarbonyl-Gly-Glu | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 10 | - |
benzyloxycarbonyl-Phe-NH2 | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 10 | - |
benzoyl-Gly-Arg | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 11 | - |
benzyloxycarbonyl-Phe-NH2 | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 15 | - |
benzyloxycarbonyl-Phe-NH2 | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 16.5 | - |
benzyloxycarbonyl-Phe-Glu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.16.5 | Saccharomyces cerevisiae | - |
baker's yeast | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.16.5 | acetyl-Phe ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.16.5 | acetyl-Phe-Leu + H2O | - |
Saccharomyces cerevisiae | acetyl-Phe + Leu | - |
? | |
| 3.4.16.5 | Acetyl-Tyr ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.16.5 | acetyl-Tyr-ethyl ester + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.16.5 | benzoyl-Gly-Arg + H2O | - |
Saccharomyces cerevisiae | benzoyl-Gly + Arg | - |
? | |
| 3.4.16.5 | benzoyl-Gly-beta-phenyllactate + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.16.5 | benzoyl-Gly-Phe + H2O | - |
Saccharomyces cerevisiae | benzoyl-Gly + Phe | - |
? | |
| 3.4.16.5 | benzoyl-L-Tyr-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | benzoyl-Tyr + 4-nitroaniline | - |
? | |
| 3.4.16.5 | benzoyl-Tyr-4-nitroanilide + H2O | - |
Saccharomyces cerevisiae | benzoyl-Tyr + 4-nitroaniline | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Ala-Ala + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Ala + Ala | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Glu-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Glu + Phe | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Glu-Tyr + H2O | - |
Saccharomyces cerevisiae | Benzyloxycarbonyl-Glu + Tyr | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Glu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Glu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Gly + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Gly-Gly-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Gly-Gly + Phe | - |
? | |
| 3.4.16.5 | Benzyloxycarbonyl-Gly-Phe + H2O | - |
Saccharomyces cerevisiae | Benzyloxycarbonyl-Gly + Phe | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-His-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-His + Phe | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-His-Tyr + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-His + Tyr | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Leu-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Leu + Leu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Leu-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Leu + Phe | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Ala + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Ala | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-beta-Ala + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + beta-Ala | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Glu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Glu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Gly + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Gly | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-His + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + His | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Leu + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Leu | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-NH2 + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Phe + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Phe | - |
? | |
| 3.4.16.5 | benzyloxycarbonyl-Phe-Pro + H2O | - |
Saccharomyces cerevisiae | benzyloxycarbonyl-Phe + Pro | - |
? | |
| 3.4.16.5 | additional information | overview: synthetic substrates | Saccharomyces cerevisiae | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.16.5 | 0.32 | - |
Benzyloxycarbonyl-Gly-Glu | pH 4.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.46 | - |
Benzyloxycarbonyl-Gly-Glu | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 0.72 | - |
Benzyloxycarbonyl-His-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.44 | - |
benzoyl-Gly-Arg | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 1.82 | - |
Benzyloxycarbonyl-His-Phe | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 2.05 | - |
Benzyloxycarbonyl-His-Phe | pH 7.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 2.18 | - |
benzyloxycarbonyl-Gly-Gly | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 2.2 | - |
Benzyloxycarbonyl-Gly-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 2.2 | - |
benzyloxycarbonyl-Phe-NH2 | pH 7.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 2.8 | - |
benzyloxycarbonyl-Phe-NH2 | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 3 | - |
benzyloxycarbonyl-Phe-His | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 3.8 | - |
benzoyl-Gly-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 5.3 | - |
benzyloxycarbonyl-Phe-NH2 | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 6.85 | - |
benzyloxycarbonyl-Leu-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 8.2 | - |
benzyloxycarbonyl-His-Tyr | pH 6.5 or pH 7.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 10.7 | - |
benzyloxycarbonyl-His-Tyr | pH 5.7, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 11.6 | - |
benzyloxycarbonyl-Phe-His | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 13.3 | - |
benzyloxycarbonyl-Glu-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 15.5 | - |
benzyloxycarbonyl-Glu-Phe | pH 4.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 16.8 | - |
benzyloxycarbonyl-Glu-Phe | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 18.8 | - |
Benzyloxycarbonyl-Glu-Tyr | pH 5.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 19 | - |
Benzyloxycarbonyl-Glu-Tyr | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 21.2 | - |
benzoyl-Gly-beta-phenyllactate | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 23 | - |
benzyloxycarbonyl-Phe-Pro | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 23.8 | - |
Benzyloxycarbonyl-Glu-Tyr | pH 6.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 24.5 | - |
benzyloxycarbonyl-Phe-Glu | pH 4.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 26.2 | - |
benzyloxycarbonyl-Phe-Glu | pH 5.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 36.1 | - |
Acetyl-Phe-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 46 | - |
benzyloxycarbonyl-Leu-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 66 | - |
benzyloxycarbonyl-Phe-beta-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 69.4 | - |
Benzyloxycarbonyl-Ala-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 96.5 | - |
benzyloxycarbonyl-Phe-Glu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 105 | - |
Acetyl-Tyr ethyl ester | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 120 | - |
benzyloxycarbonyl-Phe-Ala | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 120 | - |
acetyl-Phe ethyl ester | pH 8.0, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 122 | - |
acetyl-Phe ethyl ester | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 130 | - |
Benzyloxycarbonyl-Phe-Leu | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 140 | - |
benzyloxycarbonyl-Phe-Gly | pH 6.5, 25°C | Saccharomyces cerevisiae | |
| 3.4.16.5 | 420 | - |
benzyloxycarbonyl-Phe-Phe | pH 6.5, 25°C | Saccharomyces cerevisiae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 4.2 | - |
hydrolysis of polyglutamic acid | Saccharomyces cerevisiae |
| 3.4.16.5 | 5.5 | - |
peptides containing an acidic amino acid, e.g. benzyloxycarbonyl-Phe-Glu, and benzyloxycarbonyl-Glu-Phe | Saccharomyces cerevisiae |
| 3.4.16.5 | 6 | 7 | hydrolysis of peptides containing only neutral amino acids, e.g. benzyloxycarbonyl-Phe-Pro, benzyloxycarbonyl-Gly-Phe, benzyloxycarbonyl-Phe-Leu, benzoyl-Gly-Phe, acetyl-Phe-Leu | Saccharomyces cerevisiae |
| 3.4.16.5 | 6 | - |
hydrolysis of benzoyl-Gly-Arg | Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.16.5 | 5 | 9 | pH 5.0: about 45% of maximal activity, pH 9.0: about 75% of maximal activity, hydrolysis of acetyl-Phe ethyl ester | Saccharomyces cerevisiae |
| 3.4.16.5 | 5 | 7 | pH 5.0: about 45% of maximal activity, pH 7.0: about 50% of maximal activity, hydrolysis of hippuryl-L-beta-phenyllactate | Saccharomyces cerevisiae |
| 3.4.16.5 | 5.5 | 8.5 | pH 5.5: about 50% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of benzyloxycarbonyl-Phe-NH2 | Saccharomyces cerevisiae |
| 3.4.16.5 | 5.5 | 8 | pH 5.5: about 50% of maximal activity, pH 8.0: about 35% of maximal activity, hydrolysis of benzoyl-Tyr-4-nitroanilide | Saccharomyces cerevisiae |
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