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Literature summary extracted from

  • Cho, H.Y.; Tanizawa, K.; Soda, K.
    Role of divalent metal ions on activity and stability of thermostable dipeptidase from Bacillus stearothermophilus (1997), Biosci. Biotechnol. Biochem., 61, 1688-1692.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.13.19 substitution of Co2+ or Mn2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.19 Ba2+
-
 Geobacillus stearothermophilus
3.4.13.19 Ca2+
-
 Geobacillus stearothermophilus
3.4.13.19 Cu2+
-
 Geobacillus stearothermophilus
3.4.13.19 Hg2+
-
 Geobacillus stearothermophilus
3.4.13.19 Mg2+
-
 Geobacillus stearothermophilus
3.4.13.19 Zn2+ slight Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.19 Cd2+ activates Geobacillus stearothermophilus
3.4.13.19 Co2+ activates,substitution of Co2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus
3.4.13.19 Mn2+ activates Geobacillus stearothermophilus
3.4.13.19 Mn2+ the Mn-reconstituted enzyme has nearly twice the activity of the original Zn-enzyme, substitution of Mn2+ for Zn2+ considerably loweres the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus
3.4.13.19 Zinc the metalloenzyme contains 1 gatom of zinc per mol of subunit, the prosthetic Zn plays dual roles in conformational stability and catalysis of the thermostable dipeptidase Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.19 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.19
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.13.19 additional information
-
 substitution of Co2+ or Mn2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus
3.4.13.19 70
-
 30 min, about 15% loss of activity of the native enzyme, about 55% of the activity of the Co2+-reconstituted enzyme, about 75% loss of activity of the Mn2+-reconstituted enzyme Geobacillus stearothermophilus