Literature summary extracted from
Rybina, I.V.; Feinmark, S.J.
Alteration of human leukotriene A4 hydrolase activity after site-directed mutagenesis: serine-415 is a regulatory residue (1999), Biochim. Biophys. Acta, 1438, 199-203.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.3.2.6 |
medicine |
the lipid metabolic activity of enzyme plays a central role in the control of polymorphonuclear leukocyte function and in the development of inflammation, the regulation of enzyme presents a novel potential target for anti-inflammatory therapy |
Homo sapiens |
3.3.2.6 |
medicine |
the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 |
Homo sapiens |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.3.2.6 |
wild-type and mutant enzymes expressed in Escherichia coli |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.3.2.6 |
S415D |
significantly reduced epoxide hydrolase activity, little effect on aminopeptidase activity |
Homo sapiens |
3.3.2.6 |
S415E |
significantly reduced epoxide hydrolase activity, little effect on aminopeptidase activity |
Homo sapiens |
3.3.2.6 |
S415Q |
no effect on enzyme activity |
Homo sapiens |
3.3.2.6 |
S415T |
little effect on enzyme activity |
Homo sapiens |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.3.2.6 |
69000 |
- |
x * 69000, SDS-PAGE, mutant enzymes |
Homo sapiens |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.3.2.6 |
leukotriene A4 + H2O |
Homo sapiens |
biosynthesis of leukotriene B4 |
leukotriene B4 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.3.2.6 |
Homo sapiens |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.3.2.6 |
leukotriene A4 + H2O = leukotriene B4 |
enzyme contains two partly overlapping active sites which influence each other |
Homo sapiens |
|
3.3.2.6 |
leukotriene A4 + H2O = leukotriene B4 |
Ser415 plays a key role in regulating epoxid hydrolase activity |
Homo sapiens |
|
3.3.2.6 |
leukotriene A4 + H2O = leukotriene B4 |
bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase |
Homo sapiens |
|
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
3.3.2.6 |
epithelium |
inactive form of enzyme |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.3.2.6 |
leukotriene A4 + H2O |
- |
Homo sapiens |
leukotriene B4 |
- |
? |
|
3.3.2.6 |
leukotriene A4 + H2O |
biosynthesis of leukotriene B4 |
Homo sapiens |
leukotriene B4 |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.3.2.6 |
? |
x * 69000, SDS-PAGE, mutant enzymes |
Homo sapiens |