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Literature summary extracted from

  • Wetterholm, A.; Medina, J.F.; Radmark, O.; Shapiro, R.; Haeggström, J.Z.; Vallee, B.L.; Samuelsson, B.
    Recombinant mouse leukotriene A4 hydrolase: a zinc metalloenzyme with dual enzymatic activities (1991), Biochim. Biophys. Acta, 1080, 96-102.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.6 medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.6 expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.6 additional information
-
 additional information
-
 Mus musculus
3.3.2.6 0.005
-
 leukotriene A4 pH 8.0, 0°C Mus musculus
3.3.2.6 0.68
-
 L-alanine-4-nitroanilide pH 8.0, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.3.2.6 Co2+ apoenzyme is inactive and can be reactivated by addition of stoichiometric amounts of zinc and cobalt Mus musculus
3.3.2.6 Zinc apoenzyme is inactive and can be reactivated by addition of stoichiometric amounts of zinc and cobalt Mus musculus
3.3.2.6 Zinc zinc content: 1 mol of zinc per mol of enzyme Mus musculus
3.3.2.6 Zinc zinc protein Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.6 69000
-
 x * 69000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.6 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.6 recombinant enzyme, expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.6 leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Mus musculus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.6 0.185
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.6 L-alanine-4-nitroanilide + H2O
-
 Mus musculus L-alanine + 4-nitroaniline
-
 ?
3.3.2.6 leukotriene A4 + H2O
-
 Mus musculus leukotriene B4
-
 ?
3.3.2.6 additional information
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.6 ? x * 69000, SDS-PAGE Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.3.2.6 Mus musculus chromatofocusing
-
 5.5