Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hazra, T.K.; Izumi, T.; Boldogh, I.; Imhoff, B.; Kow, Y.W.; Jaruga, P.; Dizdaroglu, M.; Mitra, S.
    Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA (2002), Proc. Natl. Acad. Sci. USA, 99, 3523-3528.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.23 NEH1 is cloned, sequenced and expressed in Escherichia coli BL21(DE3), chromosomal location: 15q25 Homo sapiens
3.2.2.23 NEH2, chromosomal location: 4q35 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.23 additional information NEH1 is not localized in mitochondria Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 additional information no zinc finger Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.23 36800
-
 x * 36800, NEH2, calculated from the amino acid sequence Homo sapiens
3.2.2.23 43582
-
 x * 43582, recombinant NEH1, calculated from the amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA + H2O Homo sapiens involved in replication-associated repair of oxidized bases ?
-
 ?
3.2.2.23 DNA + H2O Homo sapiens DNA base excision repair enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Homo sapiens Q969S2 human nei homolog NEH2
-
3.2.2.23 Homo sapiens Q96FI4 human nei homolog NEH1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23 recombinant NEH1, expressed in Escherichia coli BL21(DE3) Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.23 brain moderate expression of NEH1 Homo sapiens
-
3.2.2.23 leukocyte low expression of NEH1 Homo sapiens
-
3.2.2.23 liver highest expression of NEH1 in liver, pancreas and thymus Homo sapiens
-
3.2.2.23 MRC-5 cell primary human diploid fibroblasts, S phase-specific expression of NEH1 Homo sapiens
-
3.2.2.23 ovary moderate expression of NEH1 Homo sapiens
-
3.2.2.23 pancreas highest expression of NEH1 in liver, pancreas and thymus Homo sapiens
-
3.2.2.23 prostate moderate expression of NEH1 Homo sapiens
-
3.2.2.23 spleen moderate expression of NEH1 Homo sapiens
-
3.2.2.23 testis low expression of NEH1 Homo sapiens
-
3.2.2.23 thymus highest expression of NEH1 in liver, pancreas and thymus Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O substrate specificity Homo sapiens ?
-
 ?
3.2.2.23 DNA + H2O with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions Homo sapiens ?
-
 ?
3.2.2.23 DNA + H2O excises formamidopyrimidines from damaged DNA and oxidized pyrimidines and 8-oxoguanine from oligodeoxynucleotides, role of the N-terminal Pro as its active site Homo sapiens ?
-
 ?
3.2.2.23 DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Homo sapiens ?
-
 ?
3.2.2.23 DNA + H2O involved in replication-associated repair of oxidized bases Homo sapiens ?
-
 ?
3.2.2.23 DNA + H2O DNA base excision repair enzyme Homo sapiens ?
-
 ?
3.2.2.23 DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O FapyGua Homo sapiens 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O FapyAde Homo sapiens 4,6-diamino-5-formamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 5-hydroxy-2'-deoxyuridine + H2O dublex oligodeoxynucleotide containing 5-hydroxyuracil when paired with G Homo sapiens ?
-
 ?
3.2.2.23 DNA containing 7-hydro-8-oxoguanine residues + H2O excises 8-oxoguanine from 31mer oligodeoxynucleotide, most active with G, followed by T, opposite the lesion, weak activity with C or A opposite Homo sapiens DNA + 7-hydro-8-oxoguanine
-
 ?
3.2.2.23 DNA containing dihydrouracil residues + H2O active as a DNA glycosylase/AP lyase with dihydrouracil 31mer dublex oligo substrate, similar activity with A, C or G opposite the lesion, reduced with T opposite Homo sapiens ?
-
 ?
3.2.2.23 additional information not: DNA containing 2-hydroxyadenine, 1-N6 ethenoadenine, 3-N4 ethenocytosine, hypoxanthine, xanthine Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.23 ? x * 36800, NEH2, calculated from the amino acid sequence Homo sapiens
3.2.2.23 ? x * 43582, recombinant NEH1, calculated from the amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.2.23 NEH1 human homolog to MutM and Nei Homo sapiens
3.2.2.23 NEH2 human homolog to MutM and Nei Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.4
-
 assay at Homo sapiens