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Literature summary extracted from

  • D'Ham, C.; Romieu, A.; Jaquinod, M.; Gasparutto, D.; Cadet, J.
    Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins: Kinetic and mechanistic aspects (1999), Biochemistry, 38, 3335-3344.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.23 0.00045
-
 DNA containing 5,6-dihydrothymine residues pH 7.5, 37°C Escherichia coli
3.2.2.23 0.0047
-
 DNA containing 5-hydroxycytosine residues pH 7.5, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.23 Zn2+ possesses a zinc finger Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.23 DNA + H2O Escherichia coli DNA base excision repair enzyme ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O Escherichia coli dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair 8-hydroxyguanine + DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.23 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.23
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.2.23 DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.23 DNA + H2O enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity Escherichia coli ?
-
 ?
3.2.2.23 DNA + H2O DNA base excision repair enzyme Escherichia coli ?
-
 ?
3.2.2.23 DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O FapyGua Escherichia coli 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O FapyAde Escherichia coli 4,6-diamino-5-formamidopyrimidine + DNA
-
 ?
3.2.2.23 DNA containing 5,6-dihydrothymine residues + H2O dublex 33mer oligonucleotide, poor substrate Escherichia coli ?
-
 ?
3.2.2.23 DNA containing 5-hydroxycytosine residues + H2O dublex 33mer oligonucleotide, excision mechanism Escherichia coli ?
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O 7,8-dihydro-8-oxoguanine-DNA Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing 8-hydroxyguanine residues + H2O dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair Escherichia coli 8-hydroxyguanine + DNA
-
 ?
3.2.2.23 DNA containing ring-opened N7-methylguanine residues + H2O
-
 Escherichia coli 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.23 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.23 7.5
-
 assay at Escherichia coli