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Literature summary extracted from

  • Yebra, M.J.; Arroyo, J.; Sanz, P.; Prieto, J.A.
    Characterization of novel neopullulanase from Bacillus polymyxa (1997), Appl. Biochem. Biotechnol., 68, 113-120.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.135 58000
-
SDS-PAGE Paenibacillus polymyxa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.135 pullulan + H2O Paenibacillus polymyxa
-
panose + ?
-
?
3.2.1.135 pullulan + H2O Paenibacillus polymyxa CECT 155
-
panose + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.135 Paenibacillus polymyxa
-
CECT 155
-
3.2.1.135 Paenibacillus polymyxa CECT 155
-
CECT 155
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.135 culture condition:pullulan-grown cell in absence of D-glucose Paenibacillus polymyxa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.135 pullulan + H2O
-
Paenibacillus polymyxa panose + ?
-
?
3.2.1.135 pullulan + H2O
-
Paenibacillus polymyxa CECT 155 panose + ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.135 50
-
-
Paenibacillus polymyxa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.135 30 70 30°C: about 70% of activity maximum, 70°C: about 50% of activity maximum Paenibacillus polymyxa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.135 50
-
30 min, pH 6.0, stable Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.135 6
-
-
Paenibacillus polymyxa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.135 5 8 pH 5 and 8: more than 50% activity Paenibacillus polymyxa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.135 6
-
30 min, 50°C, stable Paenibacillus polymyxa