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Literature summary extracted from
- New type of pullulanase from Bacillus stearothermophilus and molecular cloning and expression of the gene in Bacillus subtilis (1988), J. Bacteriol., 170, 1554-1559.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.135 | expression in Bacillus subtilis | Geobacillus stearothermophilus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | 62000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.135 | pullulan + H2O | Geobacillus stearothermophilus | - |
panose + ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.135 | Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.135 | - |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | additional information | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.135 | additional information | enzyme hydrolyzes starch | Geobacillus stearothermophilus | ? | - |
? | |
| 3.2.1.135 | pullulan + H2O | - |
Geobacillus stearothermophilus | panose + ? | - |
? | |
| 3.2.1.135 | pullulan + H2O | - |
Geobacillus stearothermophilus | panose + ? | + maltose + D-glucose, in a molar ratio of 3:1:1, small amount | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | 60 | - |
- |
Geobacillus stearothermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | 40 | 80 | 40°C: about 50% of activity maximum, 80°C: about 25% of activity maximum | Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | additional information | - |
EDTA stabilizes against thermal inactivation | Geobacillus stearothermophilus |
| 3.2.1.135 | 60 | - |
60 min, about 10% loss of activity | Geobacillus stearothermophilus |
| 3.2.1.135 | 70 | - |
60 min, about 80% loss of activity | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | 6 | - |
- |
Geobacillus stearothermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.135 | 5 | 7 | pH 5: about 25% of activity maximum, pH 7: about 40% of activity maximum | Geobacillus stearothermophilus |
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