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Literature summary extracted from

  • Alfonso, C.; Martinez, M.J.; Reyes, F.
    Purification and properties of two endochitosanases from Mucor rouxii implicated in its cell wall degradation (1992), FEMS Microbiol. Lett., 95, 187-194.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 acetate enzyme A, enzyme B is not affected Amylomyces rouxii
3.2.1.132 chitosan substrate inhibition, enzyme B Amylomyces rouxii
3.2.1.132 Cu2+
-
 Amylomyces rouxii
3.2.1.132 Hg2+
-
 Amylomyces rouxii
3.2.1.132 Mn2+
-
 Amylomyces rouxii
3.2.1.132 Pb2+
-
 Amylomyces rouxii
3.2.1.132 Sn2+
-
 Amylomyces rouxii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information
-
 Amylomyces rouxii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ activates enzyme B Amylomyces rouxii
3.2.1.132 K+ activates enzyme A Amylomyces rouxii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 63000
-
 enzyme B, gel filtration Amylomyces rouxii
3.2.1.132 79000
-
 enzyme A, gel filtration Amylomyces rouxii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Amylomyces rouxii
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Amylomyces rouxii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 2 forms: A and B Amylomyces rouxii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 42.7
-
 after second Mono Q HR 5/5 chromatography, enzyme A Amylomyces rouxii
3.2.1.132 102.3
-
 after Superose 6 HR 10/30 chromatography, enzyme B Amylomyces rouxii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Amylomyces rouxii ?
-
 ?
3.2.1.132 chitosan + H2O hydrolysis of: chitosan extracted from Mucor rouxii cell walls Amylomyces rouxii additional information mixture of dimer, trimer and higher molecular weight oligomers of glucosamine ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
 enzyme B Amylomyces rouxii
3.2.1.132 55
-
 enzyme A Amylomyces rouxii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 30
-
 pH 5.0, 30 min, stable Amylomyces rouxii
3.2.1.132 40
-
 pH 5.0, 30 min, stable Amylomyces rouxii
3.2.1.132 60
-
 30 min, pH 5.0, complete inactivation Amylomyces rouxii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5
-
-
 Amylomyces rouxii