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Literature summary extracted from

  • Yabuki, M.; Uchiyama, A.; Suzuki, K.; Ando, A.; Fujii, T.
    Purification and properties of chitosanase from Bacillus circulans MH-K1 (1988), J. Gen. Appl. Microbiol., 34, 255-270.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Cu2+
-
 Niallia circulans
3.2.1.132 Hg2+
-
 Niallia circulans
3.2.1.132 Ni2+
-
 Niallia circulans
3.2.1.132 p-chloromercuribenzoate
-
 Niallia circulans
3.2.1.132 Zn2+
-
 Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.132 additional information
-
 additional information 0.63 mg chitosan/ml Niallia circulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Niallia circulans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 27000
-
 gel filtration Niallia circulans
3.2.1.132 32000
-
 1 * 32000, SDS-PAGE Niallia circulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Niallia circulans
-
 ?
-
 ?
3.2.1.132 chitosan + H2O Niallia circulans MH-K1
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Niallia circulans
-
 MH-K1
-
3.2.1.132 Niallia circulans MH-K1
-
 MH-K1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.132 glycoprotein
-
 Niallia circulans

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Niallia circulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.132 culture medium
-
 Niallia circulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 160
-
 after gel filtration Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 carboxymethylchitosan + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Niallia circulans MH-K1 ?
-
 ?
3.2.1.132 chitosan + H2O the smallest of the substrates is a tetramer of glucosamine Niallia circulans additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O substrates with less than 40% deacetylation are not affected by the enzyme Niallia circulans additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O most susceptible substrate is: 80% deacetylated chitosan Niallia circulans additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O hydrolysis of: glycol chitosan Niallia circulans additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O the smallest of the substrates is a tetramer of glucosamine Niallia circulans MH-K1 additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O substrates with less than 40% deacetylation are not affected by the enzyme Niallia circulans MH-K1 additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 chitosan + H2O most susceptible substrate is: 80% deacetylated chitosan Niallia circulans MH-K1 additional information mixture of dimer and trimer of glucosamine ?
3.2.1.132 additional information no activity with chitin (i.e. 100% acetylated chitosan) Niallia circulans ?
-
 ?
3.2.1.132 additional information no activity with chitin (i.e. 100% acetylated chitosan) Niallia circulans MH-K1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 32000, SDS-PAGE Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
 at pH 6.5 Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132
-
-
 stable in this range Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6.5
-
-
 Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4 8 about 20% of maximal activity at pH 4 and 8 Niallia circulans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Niallia circulans density gradient column electrofocusing and acrylamide gel electrofocusing
-
 9.2