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Literature summary extracted from

  • Bouma, C.L.; Reizer, J.; Reizer, A.; Robrish, S.A.; Thompson, J.
    6-Phospho-alpha-D-glucosidase from Fusobacterium mortiferum: Cloning, expression, and assignment to family 4 of the glycosylhydrolases (1997), J. Bacteriol., 179, 4129-4137.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.122 additional information growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis Fusobacterium mortiferum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.122 malH gene encodes maltose 6P hydrolase, a 441 residues protein, and is cloned, sequenced and expressed in Escherichia coli XLOLR Fusobacterium mortiferum

General Stability

EC Number General Stability Organism
3.2.1.122 dialysis and storage overnight results in 90% loss of activity Fusobacterium mortiferum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.122 air exposure to air rapidly inactivates Fusobacterium mortiferum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.122 Co2+ requires Fe2+, Mn2+, Co2+ or Ni2+ Fusobacterium mortiferum
3.2.1.122 Fe2+ requires Fe2+, Mn2+, Co2+ or Ni2+ Fusobacterium mortiferum
3.2.1.122 Mn2+ requires Fe2+, Mn2+, Co2+ or Ni2+ Fusobacterium mortiferum
3.2.1.122 Ni2+ requires Fe2+, Mn2+, Co2+ or Ni2+ Fusobacterium mortiferum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.122 49000
-
 x * 49000, SDS-PAGE Fusobacterium mortiferum
3.2.1.122 49718
-
 x * 49718, calculated from the amino acid sequence Fusobacterium mortiferum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.122 additional information Fusobacterium mortiferum carbohydrate metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.122 Fusobacterium mortiferum O06901 strain ATCC 25557, MalH
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.1.122 O2-sensitive enzyme, rapidly inactivated by exposure to air Fusobacterium mortiferum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.122
-
 Fusobacterium mortiferum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.122 additional information
-
-
 Fusobacterium mortiferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.122 4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
-
 Fusobacterium mortiferum alpha-D-glucose 6-phosphate + 4-methylumbelliferone fluorescent product ?
3.2.1.122 maltose 6'-phosphate + H2O broad substrate specificity Fusobacterium mortiferum D-glucose 6-phosphate + D-glucose molar ratio is 1:1 ?
3.2.1.122 additional information carbohydrate metabolism Fusobacterium mortiferum ?
-
 ?
3.2.1.122 p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
-
 Fusobacterium mortiferum alpha-D-glucose 6-phosphate + p-nitrophenol yellow product ?
3.2.1.122 trehalose 6-phosphate + H2O
-
 Fusobacterium mortiferum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.122 ? x * 49000, SDS-PAGE Fusobacterium mortiferum
3.2.1.122 ? x * 49718, calculated from the amino acid sequence Fusobacterium mortiferum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.122 MalH
-
 Fusobacterium mortiferum
3.2.1.122 More belongs to familiy 4 of the glycosylhydrolase superfamily Fusobacterium mortiferum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.122 37
-
 assay at Fusobacterium mortiferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.122 7
-
 assay at Fusobacterium mortiferum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.122 Fusobacterium mortiferum analytical isoelectric focusing
-
 4.9
3.2.1.122 Fusobacterium mortiferum amino acid sequence
-
 5.5