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Literature summary extracted from

  • Varrot, A.; Yamamoto, H.; Sekiguchi, J.; Thompson, J.; Davies, G.J.
    Crystallization and preliminary X-ray analysis of the 6-phospho-alpha-glucosidase from Bacillus subtilis (1999), Acta Crystallogr. Sect. D, 55, 1212-1214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.122 glvA gene, formerly glv1, is encoded within the maltose PEP-PTS operon, it is cloned and overexpressed in Escherichia coli, glvA encodes a 449 amino acid protein Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.122 recombinant GlvA expressed in Escherichia coli, vapour-phase diffusion using the hanging-drop method, NAD/Mn2+-dependent crystal form Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.122 Mn2+ requirement, permits formation of the active tetramer of GlvA from its inactive dimeric state Bacillus subtilis
3.2.1.122 additional information requires a divalent metal ion, e.g. Mn2+ Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.122 50513
-
 4 * 50513, native GlvA is active as tetramer Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.122 maltose 6'-phosphate + H2O Bacillus subtilis enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.122 maltose 6'-phosphate + H2O Bacillus subtilis GlvA enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system D-glucose 6-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.122 Bacillus subtilis
-
 GlvA
-
3.2.1.122 Bacillus subtilis GlvA
-
 GlvA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.122 recombinant GlvA expressed in Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.122 maltose 6'-phosphate + H2O
-
 Bacillus subtilis D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.122 maltose 6'-phosphate + H2O enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system Bacillus subtilis D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.122 maltose 6'-phosphate + H2O
-
 Bacillus subtilis GlvA D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.122 maltose 6'-phosphate + H2O enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system Bacillus subtilis GlvA D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.122 trehalose 6-phosphate + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.122 trehalose 6-phosphate + H2O
-
 Bacillus subtilis GlvA ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.122 dimer inactive state Bacillus subtilis
3.2.1.122 tetramer 4 * 50513, native GlvA is active as tetramer Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.122 GlvA
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
3.2.1.122 additional information requires a divalent metal ion, e.g. Mn2+ Bacillus subtilis
3.2.1.122 NADH requirement Bacillus subtilis