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Literature summary extracted from

  • Irwin, D.C.; Cheng, M.; Xiang, B.; Rose, J.K.C.; Wilson, D.B.
    Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca (2003), Eur. J. Biochem., 270, 3083-3091.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.120 expression in Escherichia coli Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.120 0.0024
-
 tamarind xyloglucan 50°C, pH 7.5 Thermobifida fusca
3.2.1.120 0.966
-
 xyloglucan oligosaccharide 50°C, pH 7.5 Thermobifida fusca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.120 79440
-
 x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence Thermobifida fusca
3.2.1.120 79480
-
 x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.120 additional information Thermobifida fusca enzyme allows for use of cellulose as carbon source by breaking down surrounding cellulose fibrils ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.120 Thermobifida fusca
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.120 R1-Glcbeta(1-4)Glcbeta[Xylbeta(1-6)Glcbeta(1-4)](1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + H2O = R1-Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)-R2 + 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose inverting mechanism Thermobifida fusca
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.120 additional information no substrate: carboxymethylcellulose, swollen cellulose Thermobifida fusca ?
-
 ?
3.2.1.120 additional information enzyme allows for use of cellulose as carbon source by breaking down surrounding cellulose fibrils Thermobifida fusca ?
-
 ?
3.2.1.120 tamarind xyloglucan + H2O
-
 Thermobifida fusca oligosaccharide + ?
-
 ?
3.2.1.120 xyloglucan fragment oligosaccharide + H2O
-
 Thermobifida fusca isoprimeverose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.120 ? x * 79440, mass spectroscopy, x * 79480, deduced from gene sequence Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.120 55
-
 16 h, full activity Thermobifida fusca
3.2.1.120 60
-
 16 h, 75% residual activity Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.120 7.5
-
 assay at Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.120 6 9.4
-
 Thermobifida fusca