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Literature summary extracted from

  • Kuroki, G.; Poulton, J.E.
    Isolation and characterization of multiple forms of prunasin hydrolase from black cherry (Prunus serotina Ehrh.) seeds (1987), Arch. Biochem. Biophys., 255, 19-26.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.118 2-mercaptoethanol weak inhibition Prunus serotina
3.2.1.118 castanospermine PH I and PH IIb, competitive Prunus serotina
3.2.1.118 DTT weak inhibition Prunus serotina
3.2.1.118 iodoacetamide weak inhibition Prunus serotina
3.2.1.118 iodoacetic acid weak inhibition Prunus serotina
3.2.1.118 Mandelamide weak inhibition Prunus serotina
3.2.1.118 Mandelic acid weak inhibition Prunus serotina
3.2.1.118 additional information little or no inhibition by Cu2+, Mg2+, Zn2+, Pb2+, Fe2+, Fe3+, Ag2+, no inhibition by diethyldithiocarbamate, 1,10-phenanthroline, 2,2'-dipyridyl or EDTA Prunus serotina
3.2.1.118 p-chloromercuriphenylsulfonate weak inhibition Prunus serotina
3.2.1.118 p-hydroxymandelic acid weak inhibition Prunus serotina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.118 68000
-
 PH IIb and PH I, gel filtration, SDS-PAGE Prunus serotina
3.2.1.118 69500
-
 PH IIa, SDS-PAGE Prunus serotina
3.2.1.118 140000
-
 PH IIa, gel filtration Prunus serotina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.118 (R)-prunasin + H2O Prunus serotina involved in cyanogenesis from (R)-amygdalin mandelonitrile + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.118 Prunus serotina
-
 Ehrh., black cherry
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.118 glycoprotein all isoforms Prunus serotina

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.118 PH I and PH II can be completely resolved by hydroxyapatite chromatography Prunus serotina

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.118 (R)-prunasin + H2O = D-glucose + mandelonitrile three isoforms found, PH I, PH IIa, PH IIb Prunus serotina
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.118 seed
-
 Prunus serotina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.118 (R)-prunasin + H2O
-
 Prunus serotina D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O involved in cyanogenesis from (R)-amygdalin Prunus serotina mandelonitrile + D-glucose
-
 ?
3.2.1.118 (S)-sambunigrin + H2O i.e. epimer of (R)-prunasin, poor substrate Prunus serotina D-glucose + mandelonitrile
-
 ?
3.2.1.118 2-nitrophenyl beta-D-glucopyranoside + H2O very effective substrate Prunus serotina 2-nitrophenol + D-glucose
-
 ?
3.2.1.118 4-nitrophenyl beta-D-galactopyranoside + H2O poor substrate Prunus serotina 4-nitrophenol + D-galactose
-
 ?
3.2.1.118 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Prunus serotina 4-nitrophenol + D-glucose
-
 ?
3.2.1.118 additional information no substrates are (R)-amygdalin, linamarin, (neo)linustatin, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-alpha-D-mannoside, 4-nitrophenyl-beta-D-xyloside and (S)-dhurrin Prunus serotina ?
-
 ?
3.2.1.118 salicin + H2O very poor substrate Prunus serotina D-glucose + salicyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.118 dimer 2 * 69500, SDS-PAGE Prunus serotina
3.2.1.118 monomer 1 * 68000, SDS-PAGE, PH I and PH IIb Prunus serotina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.118 5
-
 all isoforms Prunus serotina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.118 4 7 all isoforms, half maximum activity Prunus serotina

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.118 0.09
-
 castanospermine PH IIb, pH 5, 30°C Prunus serotina
3.2.1.118 0.19
-
 castanospermine PH I, pH 5, 30°C Prunus serotina