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Literature summary extracted from

  • Zhou, J.; Hartmann, S.; Shepherd, B.K.; Poulton, J.E.
    Investigation of the microheterogeneity and aglycone specificity-conferring residues of black cherry prunasin hydrolases (2002), Plant Physiol., 129, 1252-1264.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.117 AH I expressed in Pichia pastoris Prunus serotina
3.2.1.118 PH1-PH5 genes, PH2 expressed in Pichia pastoris Prunus serotina

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.117 G394D enhanced activity Prunus serotina
3.2.1.117 S216H enhanced activity Prunus serotina
3.2.1.117 S216H/G394D decreased activity Prunus serotina
3.2.1.117 S216H/Y220I enhanced activity Prunus serotina
3.2.1.117 S216H/Y220I/G394D decreased activity Prunus serotina
3.2.1.117 S220I/G394D decreased activity, EC 3.2.1.118 activity detected Prunus serotina
3.2.1.117 Y220I enhanced activity Prunus serotina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.118 62080
-
 calculated from known amino acid sequence Prunus serotina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O Prunus serotina first step of cyanogenesis from amygdalin (R)-prunasin + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O Prunus serotina involved in cyanogenesis from (R)-amygdalin mandelonitrile + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.117 Prunus serotina Q945G7
-
-
3.2.1.118 Prunus serotina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.118
-
 Prunus serotina

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.117 (R)-amygdalin + H2O = (R)-prunasin + D-glucose high similarities with glycoside hydrolase family 1 enzymes Prunus serotina
a
3.2.1.118 (R)-prunasin + H2O = D-glucose + mandelonitrile five isoforms found, PH1-PH5, high sequence similarities with glucoside hydrolase family 1 enzymes Prunus serotina
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.117 leaf
-
 Prunus serotina
-
3.2.1.117 seed
-
 Prunus serotina
-
3.2.1.118 leaf
-
 Prunus serotina
-
3.2.1.118 seed
-
 Prunus serotina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O
-
 Prunus serotina (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O first step of cyanogenesis from amygdalin Prunus serotina (R)-prunasin + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O
-
 Prunus serotina D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O involved in cyanogenesis from (R)-amygdalin Prunus serotina mandelonitrile + D-glucose
-
 ?