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Literature summary extracted from

  • Brimer, L.; Cicalini, A.R.; Federici, F.; Petruccioli, M.
    Amygdalin degradation by Mucor circinelloides and Penicillium aurantiogriseum: mechanisms of hydrolysis (1998), Arch. Microbiol., 169, 106-112.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.117 extracellular
-
 Penicillium aurantiogriseum
-
-
3.2.1.117 extracellular
-
 Mucor circinelloides
-
-
3.2.1.118 extracellular
-
 Penicillium aurantiogriseum
-
-
3.2.1.118 extracellular
-
 Mucor circinelloides
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.117 58800
-
 PG I, SDS-PAGE Penicillium aurantiogriseum
3.2.1.117 66400
-
 PG II, SDS-PAGE Penicillium aurantiogriseum
3.2.1.118 58800
-
 PG I, SDS-PAGE Penicillium aurantiogriseum
3.2.1.118 66400
-
 PG II, SDS-PAGE Penicillium aurantiogriseum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O Penicillium aurantiogriseum first step of cyanogenesis from amygdalin (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O Mucor circinelloides first step of cyanogenesis from amygdalin (R)-prunasin + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O Penicillium aurantiogriseum
-
 mandelonitrile + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O Mucor circinelloides
-
 mandelonitrile + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O Prunus serotina involved in cyanogenesis from (R)-amygdalin mandelonitrile + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.117 Mucor circinelloides
-
-
-
3.2.1.117 Penicillium aurantiogriseum
-
-
-
3.2.1.118 Mucor circinelloides
-
-
-
3.2.1.118 Penicillium aurantiogriseum
-
-
-
3.2.1.118 Prunus serotina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.117
-
 Penicillium aurantiogriseum
3.2.1.117
-
 Mucor circinelloides
3.2.1.118
-
 Penicillium aurantiogriseum
3.2.1.118
-
 Mucor circinelloides

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.117 (R)-amygdalin + H2O = (R)-prunasin + D-glucose extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity Penicillium aurantiogriseum
a
3.2.1.117 (R)-amygdalin + H2O = (R)-prunasin + D-glucose extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity Mucor circinelloides
a
3.2.1.117 (R)-amygdalin + H2O = (R)-prunasin + D-glucose prunasin hydrolysis is much slower than amygdalin hydrolysis Penicillium aurantiogriseum
a
3.2.1.117 (R)-amygdalin + H2O = (R)-prunasin + D-glucose prunasin hydrolysis is much slower than amygdalin hydrolysis Mucor circinelloides
a
3.2.1.118 (R)-prunasin + H2O = D-glucose + mandelonitrile extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity, prunasin hydrolysis is much slower than amygdalin hydrolysis Penicillium aurantiogriseum
a
3.2.1.118 (R)-prunasin + H2O = D-glucose + mandelonitrile extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity, prunasin hydrolysis is much slower than amygdalin hydrolysis Mucor circinelloides
a
3.2.1.118 (R)-prunasin + H2O = D-glucose + mandelonitrile two isoforms found, PG I and PG II Mucor circinelloides
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.118 0.26
-
 purified PG I, pH 6, 25°C Penicillium aurantiogriseum
3.2.1.118 1.11
-
 purified PG II, pH 6, 25°C Penicillium aurantiogriseum
3.2.1.118 12.26
-
 purified enzyme, pH 6, 25°C Mucor circinelloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.117 (R)-amygdalin + H2O
-
 Penicillium aurantiogriseum (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O
-
 Mucor circinelloides (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O first step of cyanogenesis from amygdalin Penicillium aurantiogriseum (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-amygdalin + H2O first step of cyanogenesis from amygdalin Mucor circinelloides (R)-prunasin + D-glucose
-
 ?
3.2.1.117 (R)-prunasin + H2O
-
 Penicillium aurantiogriseum D-glucose + mandelonitrile
-
 ?
3.2.1.117 (R)-prunasin + H2O
-
 Mucor circinelloides D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O
-
 Prunus serotina D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O slow reaction Penicillium aurantiogriseum D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O slow reaction Mucor circinelloides D-glucose + mandelonitrile
-
 ?
3.2.1.118 (R)-prunasin + H2O
-
 Penicillium aurantiogriseum mandelonitrile + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O
-
 Mucor circinelloides mandelonitrile + D-glucose
-
 ?
3.2.1.118 (R)-prunasin + H2O involved in cyanogenesis from (R)-amygdalin Prunus serotina mandelonitrile + D-glucose
-
 ?