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Literature summary extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Glycoprotein processing enzymes of plants (1989), Methods Enzymol., 179, 452-475.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.113 Triton X-100 activation Vigna radiata var. radiata
3.2.1.113 Triton X-100 0.1% Vigna radiata var. radiata
3.2.1.114 Triton X-100 activation, 0.15-0.2% Vigna radiata

General Stability

EC Number General Stability Organism
3.2.1.114 glycerol stabilizes Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.113 1,4-Dideoxy-1,4-imino-D-mannitol not Vigna radiata var. radiata
3.2.1.113 1-deoxymannojirimycin
-
 Vigna radiata var. radiata
3.2.1.113 alpha1,2-Linked oligosaccharides
-
 Vigna radiata var. radiata
3.2.1.113 alpha1,3-Linked oligosaccharides
-
 Vigna radiata var. radiata
3.2.1.113 EDTA Ca2+ restores Vigna radiata var. radiata
3.2.1.113 mannosamine
-
 Vigna radiata var. radiata
3.2.1.113 additional information no inhibition by mannose, alpha1,6-linked mannose oligosaccharide, castanospermine, deoxynojirimycin, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine Vigna radiata var. radiata
3.2.1.114 1,4-Dideoxy-1,4-imino-D-mannitol
-
 Vigna radiata
3.2.1.114 Cu2+
-
 Vigna radiata
3.2.1.114 additional information not inhibited by castanospermine, deoxynojirimycin; not inhibited by deoxymannojirimycin Vigna radiata
3.2.1.114 swainsonine
-
 Vigna radiata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.113 membrane
-
 Vigna radiata var. radiata 16020
-
3.2.1.113 microsome
-
 Vigna radiata var. radiata
-
-
3.2.1.114 microsome
-
 Vigna radiata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.113 Ca2+ activation Vigna radiata var. radiata
3.2.1.113 Ca2+ 5 mM Vigna radiata var. radiata
3.2.1.113 Mg2+ activation Vigna radiata var. radiata
3.2.1.113 Mg2+ slight Vigna radiata var. radiata
3.2.1.113 Mn2+
-
 Vigna radiata var. radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 128000
-
 x * 128000, SDS-PAGE Vigna radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Vigna radiata var. radiata
-
 (Man)5GlcNAc + 4 D-mannose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.113 Vigna radiata var. radiata
-
-
-
3.2.1.114 Vigna radiata
-
 mung bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.113
-
 Vigna radiata var. radiata
3.2.1.114
-
 Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.114 seedling
-
 Vigna radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 additional information
-
-
 Vigna radiata var. radiata
3.2.1.114 additional information
-
-
 Vigna radiata

Storage Stability

EC Number Storage Stability Organism
3.2.1.113 4°C, in buffer with 10% glycerol, a few days, at early stage of purification at least 2 weeks Vigna radiata var. radiata
3.2.1.114 0-4°C, 10 mM HEPES buffer, pH 7.1, 0.1% Triton X-100, 0.5 mM PMSF, 0.2 mM dithiothreitol, 10% glycerol, at least 2 weeks, stable Vigna radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Vigna radiata var. radiata (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O removes all 4 alpha1,2-linked mannosyl residues Vigna radiata var. radiata (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.114 (Man)3GlcNAc + H2O poor substrate Vigna radiata ?
-
 ?
3.2.1.114 GlcNAc(Man)5GlcNAc + 2 H2O strict substrate specificity Vigna radiata GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose final product, via GlcNAc(Man)4GlcNAc ?
3.2.1.114 additional information not: Glc3(Man)9GlcNAc Vigna radiata ?
-
 ?
3.2.1.114 additional information not: (Man)9GlcNAc Vigna radiata ?
-
 ?
3.2.1.114 additional information not: (Man)5GlcNAc Vigna radiata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.114 ? x * 128000, SDS-PAGE Vigna radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 37
-
 assay at Vigna radiata var. radiata
3.2.1.114 37
-
 assay at Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.113 5.5 6
-
 Vigna radiata var. radiata
3.2.1.114 5.8 6.4
-
 Vigna radiata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.114 6.5
-
 below, loss of activity Vigna radiata