BRENDA - Enzyme Database

Synthesis and degradation of dolichyl phosphoryl glucose by the cellular slime mold, Dictyostelium discoideum

Crean, E.V.; Biochim. Biophys. Acta 792, 149-157 (1984)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.48
Triton X-100
-
Dictyostelium discoideum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.48
additional information
not markedly inhibited by alpha-or beta-glucosides or by nucleotides, exhibits a very low capacity to transfer glucose to endogenous oligosaccharide-lipid or protein acceptors in presence of EDTA, MgCl2 or CaCl2
Dictyostelium discoideum
3.1.4.48
PCMB
completely inhibited by 1 mM
Dictyostelium discoideum
3.1.4.48
UDP-GlcUA
most effective inhibitor, 0.025-0.050 mM, 50% inhibition
Dictyostelium discoideum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.48
Ca2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
3.1.4.48
Mg2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
3.1.4.48
Mn2+
dependent on, can activate in presence of potassium phosphate, Km for MnCl2 less than 1 mM
Dictyostelium discoideum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.48
Dictyostelium discoideum
-
cellular slime mold, strain NC-4
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.48
dolichyl beta-D-glucosyl phosphate + H2O
-
646470
Dictyostelium discoideum
dolichyl phosphate + D-glucose
-
646470
Dictyostelium discoideum
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.48
27
30
-
Dictyostelium discoideum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.48
5.5
6
-
Dictyostelium discoideum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.48
Triton X-100
-
Dictyostelium discoideum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.48
additional information
not markedly inhibited by alpha-or beta-glucosides or by nucleotides, exhibits a very low capacity to transfer glucose to endogenous oligosaccharide-lipid or protein acceptors in presence of EDTA, MgCl2 or CaCl2
Dictyostelium discoideum
3.1.4.48
PCMB
completely inhibited by 1 mM
Dictyostelium discoideum
3.1.4.48
UDP-GlcUA
most effective inhibitor, 0.025-0.050 mM, 50% inhibition
Dictyostelium discoideum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.48
Ca2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
3.1.4.48
Mg2+
less than 10% of the activation with Mn2+
Dictyostelium discoideum
3.1.4.48
Mn2+
dependent on, can activate in presence of potassium phosphate, Km for MnCl2 less than 1 mM
Dictyostelium discoideum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.48
dolichyl beta-D-glucosyl phosphate + H2O
-
646470
Dictyostelium discoideum
dolichyl phosphate + D-glucose
-
646470
Dictyostelium discoideum
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.4.48
27
30
-
Dictyostelium discoideum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.48
5.5
6
-
Dictyostelium discoideum