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Literature summary extracted from

  • Whisstock, J.C.; Romero, S.; Gurung, R.; Nandurkar, H.; Ooms, L.M.; Bottomley, S.P.; Mitchell, C.A.
    The inositol polyphosphate 5-phosphatases and the apurinic/apyrimidinic base excision repair endonucleases share a common mechanism for catalysis (2000), J. Biol. Chem., 275, 37055-37061.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.56 His-tag fusion protein expressed in Escherichia coli Mus musculus
3.1.3.56 His-tag fusion protein expressed in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.56 D232A no activity Mus musculus
3.1.3.56 D232A traces of activity Mus musculus
3.1.3.56 D384A no activity Mus musculus
3.1.3.56 D771A no activity Saccharomyces cerevisiae
3.1.3.56 E55A no activity Mus musculus
3.1.3.56 E55Q no activity Mus musculus
3.1.3.56 E631A no activity Saccharomyces cerevisiae
3.1.3.56 F392A 50% of activity Mus musculus
3.1.3.56 H184A traces of activity Mus musculus
3.1.3.56 H385A no activity Mus musculus
3.1.3.56 H730A no activity Saccharomyces cerevisiae
3.1.3.56 K386A no activity Mus musculus
3.1.3.56 N18A no activity Mus musculus
3.1.3.56 P387A traces of activity Mus musculus
3.1.3.56 T16A 100% of activity Mus musculus
3.1.3.56 V388A traces of activity Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.56 Mg2+
-
 Mus musculus
3.1.3.56 Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.56 115000
-
 SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O Mus musculus regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O Saccharomyces cerevisiae regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
4.2.99.18 DNA Mammalia
-
 fragments of DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.56 Mus musculus
-
-
-
3.1.3.56 Saccharomyces cerevisiae
-
-
-
4.2.99.18 Mammalia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.56 His-tag fusion protein expressed in Escherichia coli Mus musculus
3.1.3.56 His-tag fusion protein expressed in Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.56 1-(3-sn-phosphatidyl)-L-myo-inositol 4,5-bisphosphate + H2O
-
 Saccharomyces cerevisiae phosphatidylinositol 4-phosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Mus musculus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Saccharomyces cerevisiae D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Mus musculus D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O regulation of intracellular Ca2+ level by degradation of intracellular Ca2+ releasing second messenger D-myo-inositol 1,4,5-trisphosphate Saccharomyces cerevisiae D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
4.2.99.18 DNA
-
 Mammalia fragments of DNA
-
 ?
4.2.99.18 additional information inositol polyphosphate 5-phosphatase enzymes show high homology to AP endonucleases in regions that correspond to catalytic residues, they also share a similar mechanism of catalysis to the AP endonuclease consistent with other common functional similarities such as an absolute requirement for magnesium Mammalia ?
-
 ?