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Literature summary extracted from

  • Mitchell, C.A.; Connolly, T.M.; Majerus, P.W.
    Identification and isolation of a 75-kDa inositol polyphosphate-5-phosphatase from human platelets (1989), J. Biol. Chem., 264, 8873-8877.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.56 Inositol 1,3,4,5-tetrakisphosphate
-
 Homo sapiens
3.1.3.56 inositol 1,4-bisphosphate
-
 Homo sapiens
3.1.3.56 Mg2+ above 3 mM Homo sapiens
3.1.3.56 phosphate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.56 0.0075
-
 D-myo-Inositol 1,3,4,5-tetrakisphosphate
-
 Homo sapiens
3.1.3.56 0.016 0.019 D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.56 soluble
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.56 Ca2+ no effect Homo sapiens
3.1.3.56 Mg2+ required, maximum activity with 1-3 mM, inhibitory above 3 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.56 75000
-
 gel filtration, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O Homo sapiens
-
 D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O Homo sapiens
-
 D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.56 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.56 phosphoprotein 75000 Da enzyme not phosphorylated Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.56
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.56 platelet
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.56 25
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,3,4,5-tetrakisphosphate + H2O very poor substrate Homo sapiens D-myo-inositol 1,3,4-trisphosphate + phosphate
-
 ?
3.1.3.56 D-myo-inositol 1,4,5-trisphosphate + H2O
-
 Homo sapiens D-myo-inositol 1,4-bisphosphate + phosphate
-
 ?
3.1.3.56 inositol 1,2-cyclic 4,5-trisphosphate + H2O poor substrate Homo sapiens ?
-
 ?
3.1.3.56 additional information glycerophosphoinositol 4,5-bisphosphate is not hydrolyzed Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.56 monomer 1 * 75000, gel filtration, SDS-PAGE Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.56 0.0006
-
 Inositol 1,3,4,5-tetrakisphosphate 45000 Da enzyme Homo sapiens
3.1.3.56 0.015
-
 Inositol 1,3,4,5-tetrakisphosphate 75000 Da enzyme Homo sapiens