EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.2 | diisopropylfluorophosphate | complete inhibition | Buckleyzyma aurantiaca | |
3.1.2.2 | myristoyl-CoA | substrate inhibition, in vivo 28fold increased substrate concentration at 20°C leads to reduced enzyme activity | Buckleyzyma aurantiaca | |
3.1.2.20 | diisopropylfluorophosphate | complete inhibition | Buckleyzyma aurantiaca |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.2 | additional information | - |
additional information | Km at different temperatures | Buckleyzyma aurantiaca | |
3.1.2.2 | additional information | - |
additional information | Km for myristoyl-CoA is 59fold higher at 20°C compared to 5°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.018 | - |
myristoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.018 | - |
tetradecanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.023 | - |
palmitoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.023 | - |
hexadecanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.024 | - |
stearoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.024 | - |
octadecanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.058 | - |
palmitoleoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.058 | - |
palmitoleoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.067 | - |
decanoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.067 | - |
decanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.118 | - |
octanoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 0.118 | - |
octanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | additional information | - |
additional information | Km at different temperatures | Buckleyzyma aurantiaca | |
3.1.2.20 | 0.018 | - |
tetradecanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 0.023 | - |
hexadecanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 0.024 | - |
octadecanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 0.058 | - |
palmitoleoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 0.067 | - |
decanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 0.118 | - |
octanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 80000 | - |
1 * 80000, SDS-PAGE | Buckleyzyma aurantiaca |
3.1.2.2 | 85000 | - |
gel filtration | Buckleyzyma aurantiaca |
3.1.2.20 | 80000 | - |
1 * 80000, SDS-PAGE | Buckleyzyma aurantiaca |
3.1.2.20 | 85000 | - |
gel filtration | Buckleyzyma aurantiaca |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.2 | additional information | Buckleyzyma aurantiaca | extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest | ? | - |
? | |
3.1.2.2 | myristoyl-CoA + H2O | Buckleyzyma aurantiaca | highly preferred substrate, temperature regulated activity, mechanism | CoA + myristate | - |
? | |
3.1.2.2 | tetradecanoyl-CoA + H2O | Buckleyzyma aurantiaca | - |
CoA + tetradecanoate | - |
? | |
3.1.2.20 | additional information | Buckleyzyma aurantiaca | extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest | ? | - |
? | |
3.1.2.20 | tetradecanoyl-CoA + H2O | Buckleyzyma aurantiaca | - |
CoA + tetradecanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.2 | Buckleyzyma aurantiaca | - |
psychrophilic antarctic yeast, strain A19 | - |
3.1.2.2 | Buckleyzyma aurantiaca | - |
psychrophilic yeast, strain A19 | - |
3.1.2.20 | Buckleyzyma aurantiaca | - |
psychrophilic antarctic yeast, strain A19 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.2.2 | glycoprotein | highly glycosylated, 19% of the molecular weight is due to carbohydrates | Buckleyzyma aurantiaca |
3.1.2.2 | glycoprotein | 19% of the MW is formed by the glycosidic portion | Buckleyzyma aurantiaca |
3.1.2.20 | glycoprotein | highly glycosylated, 19% of the molecular weight is due to carbohydrates | Buckleyzyma aurantiaca |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.2 | 351fold | Buckleyzyma aurantiaca |
3.1.2.20 | 351fold | Buckleyzyma aurantiaca |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.2.2 | palmitoyl-CoA + H2O = CoA + palmitate | active site serine residue | Buckleyzyma aurantiaca | |
3.1.2.20 | an acyl-CoA + H2O = CoA + a carboxylate | active site serine residue | Buckleyzyma aurantiaca |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 108 | - |
purified enzyme | Buckleyzyma aurantiaca |
3.1.2.2 | 108 | - |
purified enzyme, substrate myristoyl-CoA | Buckleyzyma aurantiaca |
3.1.2.20 | 108 | - |
purified enzyme, substrate myristoyl-CoA | Buckleyzyma aurantiaca |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.2 | acyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + a carboxylate | - |
? | |
3.1.2.2 | decanoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + decanoate | - |
? | |
3.1.2.2 | hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Buckleyzyma aurantiaca | CoA + hexadecanoate | - |
? | |
3.1.2.2 | additional information | no activity with short-chain acyl-CoA substrates | Buckleyzyma aurantiaca | ? | - |
? | |
3.1.2.2 | additional information | extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest | Buckleyzyma aurantiaca | ? | - |
? | |
3.1.2.2 | myristoyl-CoA + H2O | highly preferred substrate | Buckleyzyma aurantiaca | CoA + myristate | - |
? | |
3.1.2.2 | myristoyl-CoA + H2O | highly preferred substrate, temperature regulated activity, mechanism | Buckleyzyma aurantiaca | CoA + myristate | - |
? | |
3.1.2.2 | octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Buckleyzyma aurantiaca | CoA + octadecanoate | - |
? | |
3.1.2.2 | octanoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + octanoate | - |
? | |
3.1.2.2 | palmitoleoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + palmitoleoate | - |
? | |
3.1.2.2 | palmitoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + palmitate | - |
? | |
3.1.2.2 | stearoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + stearate | - |
? | |
3.1.2.2 | tetradecanoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.2 | tetradecanoyl-CoA + H2O | specific for | Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.2 | tetradecanoyl-CoA + H2O | best substrate | Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.2 | tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.20 | acyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + a carboxylate | - |
? | |
3.1.2.20 | decanoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + decanoate | - |
? | |
3.1.2.20 | hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Buckleyzyma aurantiaca | CoA + hexadecanoate | - |
? | |
3.1.2.20 | additional information | extreme accumulation, 28fold increased compared to 0°C, of myristoyl-CoA in cells at nonpermissive temperature close to 20°C, causing growth arrest | Buckleyzyma aurantiaca | ? | - |
? | |
3.1.2.20 | octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Buckleyzyma aurantiaca | CoA + octadecanoate | - |
? | |
3.1.2.20 | octanoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + octanoate | - |
? | |
3.1.2.20 | palmitoleoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + palmitoleoate | - |
? | |
3.1.2.20 | tetradecanoyl-CoA + H2O | - |
Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.20 | tetradecanoyl-CoA + H2O | specific for | Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.20 | tetradecanoyl-CoA + H2O | best substrate | Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? | |
3.1.2.20 | tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Buckleyzyma aurantiaca | CoA + tetradecanoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.2.2 | monomer | 1 * 80000, SDS-PAGE | Buckleyzyma aurantiaca |
3.1.2.20 | monomer | 1 * 80000, SDS-PAGE | Buckleyzyma aurantiaca |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.2.2 | long chain acyl-CoA thioesterase | - |
Buckleyzyma aurantiaca |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 5 | - |
below, optimal growth temperature in vivo | Buckleyzyma aurantiaca |
3.1.2.2 | 45 | - |
- |
Buckleyzyma aurantiaca |
3.1.2.20 | 45 | - |
- |
Buckleyzyma aurantiaca |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | additional information | - |
organism is unable to grow at temperatures close to 20°C or above | Buckleyzyma aurantiaca |
3.1.2.2 | additional information | - |
the strain is inable to grow at temperatures close to 20°C and higher | Buckleyzyma aurantiaca |
3.1.2.20 | additional information | - |
organism is unable to grow at temperatures close to 20°C or above | Buckleyzyma aurantiaca |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 40 | - |
60 min, pH 7.0, 82% remaining activity | Buckleyzyma aurantiaca |
3.1.2.2 | 40 | - |
60 min, 82% remaining activity | Buckleyzyma aurantiaca |
3.1.2.2 | 50 | - |
60 min, pH 7.0, 52% remaining activity | Buckleyzyma aurantiaca |
3.1.2.2 | 50 | - |
60 min, 52% remaining activity | Buckleyzyma aurantiaca |
3.1.2.2 | 60 | - |
60 min, pH 7.0, 3% remaining activity | Buckleyzyma aurantiaca |
3.1.2.2 | 60 | - |
60 min, 3% remaining activity | Buckleyzyma aurantiaca |
3.1.2.20 | 40 | - |
60 min, pH 7.0, 82% remaining activity | Buckleyzyma aurantiaca |
3.1.2.20 | 50 | - |
60 min, pH 7.0, 52% remaining activity | Buckleyzyma aurantiaca |
3.1.2.20 | 60 | - |
60 min, pH 7.0, 3% remaining activity | Buckleyzyma aurantiaca |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.2 | 106 | - |
palmitoleoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 106 | - |
palmitoleoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 121 | - |
stearoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 121 | - |
stearoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 136 | - |
decanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 136 | - |
decanoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 153 | - |
palmitoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 153 | - |
palmitoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 159 | - |
octanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 159 | - |
octanoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 183 | - |
myristoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.2 | 183 | - |
myristoyl-CoA | pH 8.0, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 106 | - |
palmitoleoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 121 | - |
stearoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 136 | - |
decanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 153 | - |
palmitoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 159 | - |
octanoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca | |
3.1.2.20 | 183 | - |
myristoyl-CoA | pH 7.4, 25°C | Buckleyzyma aurantiaca |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 8 | - |
- |
Buckleyzyma aurantiaca |
3.1.2.20 | 8 | - |
- |
Buckleyzyma aurantiaca |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 5 | 10 | - |
Buckleyzyma aurantiaca |
3.1.2.2 | 5 | 10 | active and stable | Buckleyzyma aurantiaca |
3.1.2.20 | 5 | 10 | active and stable | Buckleyzyma aurantiaca |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.2.2 | Buckleyzyma aurantiaca | - |
- |
4.4 |
3.1.2.2 | Buckleyzyma aurantiaca | isoelectric focusing | - |
4.4 |
3.1.2.20 | Buckleyzyma aurantiaca | - |
- |
4.4 |