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Literature summary extracted from

  • Maloberti, P.; Lozano, R.C.; Mele, P.G.; Cano, F.; Colonna, C.; Mendez, C.F.; Paz, C.; Podesta, E.J.
    Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells (2002), Eur. J. Biochem., 269, 5599-5607.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.2 ACTH
-
 Homo sapiens
3.1.2.20 ACTH
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.2 expression in Escherichia coli Homo sapiens
3.1.2.2 isozymes MTE-I, His-tagged, and CTE-I, both GST-fusion proteins Homo sapiens
3.1.2.20 expression in Escherichia coli Homo sapiens
3.1.2.20 isozymes MTE-I, His-tagged, and CTE-I, both GST-fusion proteins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 NGDA isozymes CTE-I and MTE-I Homo sapiens
3.1.2.20 NGDA isozymes CTE-I and MTE-I Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.002
-
 Arachidonoyl-CoA isozyme MTE-I, pH 7.4, 37°C Homo sapiens
3.1.2.2 0.0041
-
 Arachidonoyl-CoA isozyme CTE-I, pH 7.4, 37°C Homo sapiens
3.1.2.20 0.002
-
 Arachidonoyl-CoA isozyme MTE-I, pH 7.4, 37°C Homo sapiens
3.1.2.20 0.0041
-
 Arachidonoyl-CoA isozyme CTE-I, pH 7.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol isozyme CTE I Homo sapiens 5829
-
3.1.2.2 mitochondrion isozyme MTE I Homo sapiens 5739
-
3.1.2.20 cytosol isozyme CTE I Homo sapiens 5829
-
3.1.2.20 mitochondrion isozyme MTE I Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 arachidonoyl-CoA + H2O Homo sapiens involved in cholesterol metabolism CoA + arachidonate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O Homo sapiens enzyme is responsible for the mobilization of stored arachidonic acid in cells CoA + arachidonate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O Homo sapiens involved in cholesterol metabolism CoA + arachidonate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O Homo sapiens enzyme is responsible for the mobilization of stored arachidonic acid in cells CoA + arachidonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Homo sapiens
-
 isozymes CTE-I and MTE-I
-
3.1.2.20 Homo sapiens
-
 isozymes CTE-I and MTE-I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 recombinant GST-tagged isozymes CTE-I and MTE-I, this one also His-tagged, from Escherichia coli, to homogeneity Homo sapiens
3.1.2.20 recombinant GST-tagged isozymes CTE-I and MTE-I, this one also His-tagged, from Escherichia coli, to homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 adrenal gland isozyme CTE-I Homo sapiens
-
3.1.2.20 adrenal gland isozyme CTE-I Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 arachidonoyl-CoA + H2O
-
 Homo sapiens CoA + arachidonate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O involved in cholesterol metabolism Homo sapiens CoA + arachidonate
-
 ?
3.1.2.2 arachidonoyl-CoA + H2O enzyme is responsible for the mobilization of stored arachidonic acid in cells Homo sapiens CoA + arachidonate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O
-
 Homo sapiens CoA + arachidonate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O involved in cholesterol metabolism Homo sapiens CoA + arachidonate
-
 ?
3.1.2.20 arachidonoyl-CoA + H2O enzyme is responsible for the mobilization of stored arachidonic acid in cells Homo sapiens CoA + arachidonate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 37
-
 assay at Homo sapiens
3.1.2.20 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 7.4
-
 assay at Homo sapiens
3.1.2.20 7.4
-
 assay at Homo sapiens