EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.2 | bovine serum albumin | activation | Sus scrofa | |
3.1.2.2 | bovine serum albumin | only with long-chain acyl-CoA as substrate | Sus scrofa | |
3.1.2.20 | bovine serum albumin | activation | Sus scrofa | |
3.1.2.20 | bovine serum albumin | only with long-chain acyl-CoA as substrate | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.2 | ADP | - |
Sus scrofa | |
3.1.2.2 | AMP | acetyl-CoA as substrate | Sus scrofa | |
3.1.2.2 | ATP | competitive to acetyl-CoA | Sus scrofa | |
3.1.2.2 | citrate | - |
Sus scrofa | |
3.1.2.2 | CoA-S-S-CoA | competitive to acetyl-CoA; i.e. oxidized CoA, additive with NADH, kinetics | Sus scrofa | |
3.1.2.2 | CoASH | product inhibition, acetyl-CoA as substrate, kinetics | Sus scrofa | |
3.1.2.2 | Cu2+ | - |
Sus scrofa | |
3.1.2.2 | diethyldicarbonate | - |
Sus scrofa | |
3.1.2.2 | diisopropylfluorophosphate | - |
Sus scrofa | |
3.1.2.2 | GTP | acetyl-CoA as substrate | Sus scrofa | |
3.1.2.2 | hexadecanoyl-CoA | substrate inhibition | Sus scrofa | |
3.1.2.2 | malate | weak | Sus scrofa | |
3.1.2.2 | Mn2+ | - |
Sus scrofa | |
3.1.2.2 | additional information | no inhibition by EDTA, Mg2+, Fe2+, NAD+, NADP+, NADPH, glutamate, 2-oxoglutarate, pyruvate, alanine, asparagine, succinate; no inhibition by NEM | Sus scrofa | |
3.1.2.2 | NADH | additive with oxidized CoA, noncompetitive | Sus scrofa | |
3.1.2.2 | Ni2+ | - |
Sus scrofa | |
3.1.2.2 | PMSF | no inhibition | Sus scrofa | |
3.1.2.2 | Zn2+ | - |
Sus scrofa | |
3.1.2.20 | ADP | - |
Sus scrofa | |
3.1.2.20 | AMP | acetyl-CoA as substrate | Sus scrofa | |
3.1.2.20 | ATP | competitive to acetyl-CoA | Sus scrofa | |
3.1.2.20 | citrate | - |
Sus scrofa | |
3.1.2.20 | CoA-S-S-CoA | competitive to acetyl-CoA; i.e. oxidized CoA, additive with NADH, kinetics | Sus scrofa | |
3.1.2.20 | CoASH | product inhibition, acetyl-CoA as substrate, kinetics | Sus scrofa | |
3.1.2.20 | Cu2+ | - |
Sus scrofa | |
3.1.2.20 | diethyldicarbonate | - |
Sus scrofa | |
3.1.2.20 | diisopropylfluorophosphate | - |
Sus scrofa | |
3.1.2.20 | GTP | acetyl-CoA as substrate | Sus scrofa | |
3.1.2.20 | hexadecanoyl-CoA | substrate inhibition | Sus scrofa | |
3.1.2.20 | malate | weak | Sus scrofa | |
3.1.2.20 | Mn2+ | - |
Sus scrofa | |
3.1.2.20 | additional information | no inhibition by EDTA, Mg2+, Fe2+, NAD+, NADP+, NADPH, glutamate, 2-oxoglutarate, pyruvate, alanine, asparagine, succinate; no inhibition by NEM | Sus scrofa | |
3.1.2.20 | NADH | additive with oxidized CoA, noncompetitive | Sus scrofa | |
3.1.2.20 | Ni2+ | - |
Sus scrofa | |
3.1.2.20 | PMSF | no inhibition | Sus scrofa | |
3.1.2.20 | Zn2+ | - |
Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.2 | 0.048 | - |
acetyl-CoA | - |
Sus scrofa | |
3.1.2.2 | 0.048 | - |
acetoacetyl-CoA | - |
Sus scrofa | |
3.1.2.2 | 0.048 | - |
succinyl-CoA | - |
Sus scrofa | |
3.1.2.2 | 0.048 | - |
n-butyryl-CoA | - |
Sus scrofa | |
3.1.2.2 | 0.048 | - |
n-decanoyl-CoA | - |
Sus scrofa | |
3.1.2.20 | 0.048 | - |
acetyl-CoA | - |
Sus scrofa | |
3.1.2.20 | 0.048 | - |
acetoacetyl-CoA | - |
Sus scrofa | |
3.1.2.20 | 0.048 | - |
succinyl-CoA | - |
Sus scrofa | |
3.1.2.20 | 0.048 | - |
n-butyryl-CoA | - |
Sus scrofa | |
3.1.2.20 | 0.048 | - |
n-decanoyl-CoA | - |
Sus scrofa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.2.2 | cytosol | - |
Sus scrofa | 5829 | - |
3.1.2.2 | mitochondrion | - |
Sus scrofa | 5739 | - |
3.1.2.20 | cytosol | - |
Sus scrofa | 5829 | - |
3.1.2.20 | mitochondrion | - |
Sus scrofa | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.2.2 | Ca2+ | activation, 0.1-2 mM | Sus scrofa | |
3.1.2.20 | Ca2+ | activation, 0.1-2 mM | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 300000 | - |
gel filtration | Sus scrofa |
3.1.2.20 | 300000 | - |
gel filtration | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.2 | hexadecanoyl-CoA + H2O | Sus scrofa | reaction at 60% the rate of acetoacetyl-CoA hydrolysis | CoA + hexadecanoate | - |
? | |
3.1.2.20 | hexadecanoyl-CoA + H2O | Sus scrofa | reaction at 60% the rate of acetoacetyl-CoA hydrolysis | CoA + hexadecanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.2.2 | Sus scrofa | - |
- |
- |
3.1.2.20 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.2.2 | partial, 44.3fold | Sus scrofa |
3.1.2.20 | partial, 44.3fold | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.2.2 | cardiac muscle | - |
Sus scrofa | - |
3.1.2.20 | cardiac muscle | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 2.7 | - |
partially purified enzyme | Sus scrofa |
3.1.2.20 | 2.7 | - |
partially purified enzyme | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.2.2 | 3-hydroxybutyryl-CoA + H2O | reaction at 4.3% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + 3-hydroxybutanoate | - |
? | |
3.1.2.2 | 3-hydroxydecanoyl-CoA + H2O | reaction at 1.4% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + 3-hydroxydecanoate | - |
? | |
3.1.2.2 | 3-oxodecanoyl-CoA + H2O | - |
Sus scrofa | CoA + 3-oxodecanoate | - |
? | |
3.1.2.2 | acetoacetyl-CoA + H2O | best substrate | Sus scrofa | CoA + acetoacetate | - |
? | |
3.1.2.2 | acetyl-CoA + H2O | reaction at 33% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + acetate | - |
? | |
3.1.2.2 | acyl-CoA + H2O | - |
Sus scrofa | CoA + a carboxylate | - |
? | |
3.1.2.2 | crotonyl-CoA + H2O | i.e. [trans]-2-butenoyl-CoA, reaction at 9.4% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + crotonate | - |
? | |
3.1.2.2 | decanoyl-CoA + H2O | reaction at 89% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + decanoate | - |
? | |
3.1.2.2 | hexadecanoyl-CoA + H2O | reaction at 60% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + hexadecanoate | - |
? | |
3.1.2.2 | additional information | no substrates are malonyl-CoA, acetoacetyl glutathione, acetoacetyl N-acetylcysteamine or acetoacetyl pantetheine | Sus scrofa | ? | - |
? | |
3.1.2.2 | n-butyryl-CoA + H2O | reaction at 81% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + n-butanoate | - |
? | |
3.1.2.2 | oleoyl-CoA + H2O | i.e. [cis]-9-octadecenoyl-CoA, reaction at 40% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + oleate | - |
? | |
3.1.2.2 | succinyl-CoA + H2O | reaction at 33% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + succinate | - |
? | |
3.1.2.20 | 3-hydroxybutyryl-CoA + H2O | reaction at 4.3% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + 3-hydroxybutanoate | - |
? | |
3.1.2.20 | 3-hydroxydecanoyl-CoA + H2O | reaction at 1.4% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + 3-hydroxydecanoate | - |
? | |
3.1.2.20 | 3-oxodecanoyl-CoA + H2O | - |
Sus scrofa | CoA + 3-oxodecanoate | - |
? | |
3.1.2.20 | acetoacetyl-CoA + H2O | best substrate | Sus scrofa | CoA + acetoacetate | - |
? | |
3.1.2.20 | acetyl-CoA + H2O | reaction at 33% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + acetate | - |
? | |
3.1.2.20 | acyl-CoA + H2O | - |
Sus scrofa | CoA + a carboxylate | - |
? | |
3.1.2.20 | crotonyl-CoA + H2O | i.e. [trans]-2-butenoyl-CoA, reaction at 9.4% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + crotonate | - |
? | |
3.1.2.20 | decanoyl-CoA + H2O | reaction at 89% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + decanoate | - |
? | |
3.1.2.20 | hexadecanoyl-CoA + H2O | reaction at 60% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + hexadecanoate | - |
? | |
3.1.2.20 | additional information | no substrates are malonyl-CoA, acetoacetyl glutathione, acetoacetyl N-acetylcysteamine or acetoacetyl pantetheine | Sus scrofa | ? | - |
? | |
3.1.2.20 | n-butyryl-CoA + H2O | reaction at 81% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + n-butanoate | - |
? | |
3.1.2.20 | oleoyl-CoA + H2O | i.e. [cis]-9-octadecenoyl-CoA, reaction at 40% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + oleate | - |
? | |
3.1.2.20 | succinyl-CoA + H2O | reaction at 33% the rate of acetoacetyl-CoA hydrolysis | Sus scrofa | CoA + succinate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 28 | - |
assay at | Sus scrofa |
3.1.2.20 | 28 | - |
assay at | Sus scrofa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | 45 | - |
5 min, in 0.02 M Tris-buffer, pH 8.0, 15-19% loss of activity, 10 min, 35-38% loss of activity | Sus scrofa |
3.1.2.2 | 47.5 | - |
5 min, in 0.02 M Tris-buffer, pH 8.0, 35-39% loss of activity | Sus scrofa |
3.1.2.2 | 50 | - |
5 min, in 0.02 M Tris-buffer, pH 8.0, 80% loss of activity | Sus scrofa |
3.1.2.20 | 45 | - |
5 min, in 0.02 M Tris-buffer, pH 8.0, 15-19% loss of activity, 10 min, 35-38% loss of activity | Sus scrofa |
3.1.2.20 | 47.5 | - |
5 min, in 0.02 M Tris-buffer, pH 8.0, 35-39% loss of activity | Sus scrofa |
3.1.2.20 | 50 | - |
5 min, in 0.02 M Tris-buffer, pH 8.0, 80% loss of activity | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.2.2 | additional information | - |
- |
Sus scrofa |
3.1.2.2 | 8 | - |
assay at | Sus scrofa |
3.1.2.20 | additional information | - |
- |
Sus scrofa |
3.1.2.20 | 8 | - |
assay at | Sus scrofa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.2.2 | 0.008 | - |
CoA | pH 8.0, 28°C, versus acetyl-CoA | Sus scrofa | |
3.1.2.2 | 0.052 | - |
ATP | pH 8.0, 28°C, versus acetyl-CoA | Sus scrofa | |
3.1.2.2 | 0.053 | - |
CoA-S-S-CoA | pH 8.0, 28°C, versus acetyl-CoA | Sus scrofa | |
3.1.2.2 | 0.085 | - |
CoA-S-S-CoA | pH 8.0, 28°C, with palmitoyl-CoA as substrate | Sus scrofa | |
3.1.2.2 | 0.5 | - |
NADH | pH 8.0, 28°C, with palmitoyl-CoA as substrate | Sus scrofa | |
3.1.2.20 | 0.008 | - |
CoA | pH 8.0, 28°C, versus acetyl-CoA | Sus scrofa | |
3.1.2.20 | 0.052 | - |
ATP | pH 8.0, 28°C, versus acetyl-CoA | Sus scrofa | |
3.1.2.20 | 0.053 | - |
CoA-S-S-CoA | pH 8.0, 28°C, versus acetyl-CoA | Sus scrofa | |
3.1.2.20 | 0.085 | - |
CoA-S-S-CoA | pH 8.0, 28°C, with palmitoyl-CoA as substrate | Sus scrofa | |
3.1.2.20 | 0.5 | - |
NADH | pH 8.0, 28°C, with palmitoyl-CoA as substrate | Sus scrofa |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.2.2 | Sus scrofa | - |
- |
4.5 |
3.1.2.20 | Sus scrofa | - |
- |
4.5 |