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Literature summary extracted from

  • Kurooka, S.; Hosoki, K.; Yoshimura, Y.
    Some properties of long fatty acyl-coenzyme A thioesterase in rat organs (1972), J. Biochem., 71, 625-634.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.00013 0.006 fatty-acyl-CoA thioester
-
 Rattus norvegicus
3.1.2.20 0.00013 0.006 fatty-acyl-CoA thioester
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.2 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.2 microsome
-
 Rattus norvegicus
-
-
3.1.2.2 mitochondrion
-
 Rattus norvegicus 5739
-
3.1.2.20 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.20 microsome
-
 Rattus norvegicus
-
-
3.1.2.20 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 50000
-
 sucrose density gradient centrifugation Rattus norvegicus
3.1.2.2 100000
-
 gel filtration Rattus norvegicus
3.1.2.20 50000
-
 sucrose density gradient centrifugation Rattus norvegicus
3.1.2.20 100000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Rattus norvegicus
-
-
-
3.1.2.20 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 40-60fold Rattus norvegicus
3.1.2.20 40-60fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.2 brain
-
 Rattus norvegicus
-
3.1.2.2 erythrocyte
-
 Rattus norvegicus
-
3.1.2.2 heart
-
 Rattus norvegicus
-
3.1.2.2 kidney
-
 Rattus norvegicus
-
3.1.2.2 liver
-
 Rattus norvegicus
-
3.1.2.2 spleen
-
 Rattus norvegicus
-
3.1.2.2 testis
-
 Rattus norvegicus
-
3.1.2.20 brain
-
 Rattus norvegicus
-
3.1.2.20 erythrocyte
-
 Rattus norvegicus
-
3.1.2.20 heart
-
 Rattus norvegicus
-
3.1.2.20 kidney
-
 Rattus norvegicus
-
3.1.2.20 liver
-
 Rattus norvegicus
-
3.1.2.20 spleen
-
 Rattus norvegicus
-
3.1.2.20 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 0.26
-
-
 Rattus norvegicus
3.1.2.20 0.26
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.2.2 -20°C over 1 month Rattus norvegicus
3.1.2.20 -20°C over 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Rattus norvegicus CoA + a carboxylate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.2 37
-
-
 Rattus norvegicus
3.1.2.20 37
-
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8 8.5
-
 Rattus norvegicus
3.1.2.20 8 8.5
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7.5 9
-
 Rattus norvegicus
3.1.2.20 7.5 9
-
 Rattus norvegicus