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Literature summary extracted from

  • Bonner, W.M.; Bloch, K.
    Purification and properties of fatty acyl thioesterase I from Escherichia coli (1972), J. Biol. Chem., 247, 3123-3133.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.2 2,4-dinitrofluorobenzene inhibits very slowly Escherichia coli
3.1.2.2 iodoacetamide
-
 Escherichia coli
3.1.2.2 methylene blue photooxidation Escherichia coli
3.1.2.20 2,4-dinitrofluorobenzene inhibits very slowly Escherichia coli
3.1.2.20 iodoacetamide
-
 Escherichia coli
3.1.2.20 methylene blue photooxidation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.2 0.00013 0.006 fatty-acyl-CoA thioester
-
 Escherichia coli
3.1.2.20 0.00013 0.006 fatty-acyl-CoA thioester
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.2 30000
-
 4 * 30000, SDS-PAGE Escherichia coli
3.1.2.2 122000
-
 gel filtration, thioesterase I Escherichia coli
3.1.2.20 30000
-
 4 * 30000, SDS-PAGE Escherichia coli
3.1.2.20 122000
-
 gel filtration, thioesterase I Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.2 palmitoyl-CoA + H2O Escherichia coli
-
 CoA + palmitate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O Escherichia coli
-
 CoA + palmitate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.2 Escherichia coli
-
-
-
3.1.2.20 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.2 8400fold Escherichia coli
3.1.2.20 8400fold Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.2 320
-
-
 Escherichia coli
3.1.2.20 320
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.2 acyl-CoA + H2O long-chain fatty-acyl-CoA Escherichia coli CoA + a carboxylate
-
 ?
3.1.2.2 decanoyl-CoA + H2O
-
 Escherichia coli CoA + decanoate
-
 ?
3.1.2.2 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Escherichia coli CoA + hexadecanoate
-
 ?
3.1.2.2 hexanoyl-CoA + H2O
-
 Escherichia coli CoA + hexanoate
-
 ?
3.1.2.2 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Escherichia coli CoA + octadecanoate
-
 ?
3.1.2.2 oleoyl pantetheine + H2O
-
 Escherichia coli oleate + pantetheine
-
 ?
3.1.2.2 oleoyl-CoA + H2O
-
 Escherichia coli CoA + oleate
-
 ?
3.1.2.2 oleyl-[acyl-carrier protein] + H2O
-
 Escherichia coli oleate + acyl-carrier protein
-
 ?
3.1.2.2 palmitoleoyl-CoA + H2O
-
 Escherichia coli CoA + palmitoleoate
-
 ?
3.1.2.2 palmitoyl-CoA + H2O
-
 Escherichia coli CoA + palmitate
-
 ?
3.1.2.2 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Escherichia coli CoA + tetradecanoate
-
 ?
3.1.2.2 vaccenoyl-CoA + H2O cis-isomer Escherichia coli CoA + vaccenoate
-
 ?
3.1.2.20 acyl-CoA + H2O long-chain fatty-acyl-CoA Escherichia coli CoA + a carboxylate
-
 ?
3.1.2.20 decanoyl-CoA + H2O
-
 Escherichia coli CoA + decanoate
-
 ?
3.1.2.20 hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Escherichia coli CoA + hexadecanoate
-
 ?
3.1.2.20 hexanoyl-CoA + H2O
-
 Escherichia coli CoA + hexanoate
-
 ?
3.1.2.20 octadecanoyl-CoA + H2O i.e. stearoyl-CoA Escherichia coli CoA + octadecanoate
-
 ?
3.1.2.20 oleoyl pantetheine + H2O
-
 Escherichia coli oleate + pantetheine
-
 ?
3.1.2.20 oleoyl-CoA + H2O
-
 Escherichia coli CoA + oleate
-
 ?
3.1.2.20 oleyl-[acyl-carrier protein] + H2O
-
 Escherichia coli oleate + acyl-carrier protein
-
 ?
3.1.2.20 palmitoleoyl-CoA + H2O
-
 Escherichia coli CoA + palmitoleoate
-
 ?
3.1.2.20 palmitoyl-CoA + H2O
-
 Escherichia coli CoA + palmitate
-
 ?
3.1.2.20 tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Escherichia coli CoA + tetradecanoate
-
 ?
3.1.2.20 vaccenoyl-CoA + H2O cis-isomer Escherichia coli CoA + vaccenoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.2 tetramer 4 * 30000, SDS-PAGE Escherichia coli
3.1.2.20 tetramer 4 * 30000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.2 8.4
-
-
 Escherichia coli
3.1.2.20 8.4
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.2.2 7 9.8
-
 Escherichia coli
3.1.2.20 7 9.8
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.2.2 additional information
-
-
 Escherichia coli
3.1.2.20 additional information
-
-
 Escherichia coli