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Literature summary extracted from

  • Baek, S.H.; Woo, S.K.; Lee, J.I.; Yoo, Y.J.; Cho, C.M.; Kang, M.S.; Tanaka, K.; Chung, C.H.
    New de-ubiquitinating enzyme, ubiquitin C-terminal hydrolase 8, in chick skeletal muscle (1997), Biochem. J., 325, 325-330.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.12 Ca2+ 59% inhibition at 5 mM Gallus gallus
3.4.19.12 KCl 92% inhibition at 0.2 M Gallus gallus
3.4.19.12 Mg2+ 61% inhibition at 5 mM Gallus gallus
3.4.19.12 Mn2+ 98% inhibition at 5 mM Gallus gallus
3.4.19.12 additional information no inhibition by PMSF, EDTA, and o-phenanthroline Gallus gallus
3.4.19.12 N-ethylmaleimide UCH-8 Gallus gallus
3.4.19.12 Ubiquitin aldehyde UCH-8 Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.19.12 130000
-
 2 * 130000, reducing SDS-PAGE Gallus gallus
3.4.19.12 240000
-
 UCH-8, gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.12 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.12 UCH-8, to homogeneity Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.19.12 skeletal muscle pectoralis Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.12 branched polyubiquitin + H2O substrate is ligated to proteins via epsilon-NH-isopeptide bonds Gallus gallus ubiquitin
-
 ?
3.4.19.12 additional information no activity with poly-His-tagged di-ubiquitin Gallus gallus ?
-
 ?
3.4.19.12 ubiquitin-alpha-NH-dihydrofolate reductase + H2O
-
 Gallus gallus ubiquitin + dihydrofolate reductase
-
 ?
3.4.19.12 ubiquitin-alpha-NH-MHISPPEPESEEEEHYC + H2O
-
 Gallus gallus ubiquitin + MHISPPEPESEEEEHYC
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.19.12 dimer 2 * 130000, reducing SDS-PAGE Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
3.4.19.12 ubiquitin C-terminal hydrolase 8
-
 Gallus gallus
3.4.19.12 UCH
-
 Gallus gallus
3.4.19.12 UCH-8
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.19.12 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.12 7.5 8 UCH-8 Gallus gallus
3.4.19.12 7.8
-
 assay at Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.19.12 7 9 little or no activity below pH 7.0 and above pH 9.0 Gallus gallus