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Literature summary extracted from

  • Matsuo, K.; Kobayashi, K.; Hagiwara, K.; Kajii, T.
    Purification and characterization of esterases D1 and D2 from human erythrocytes. Evidence that they are monomers (1985), Eur. J. Biochem., 153, 217-222.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.56 0.03
-
 4-yethylumbelliferyl acetate pH 5.2, 37°C, isoenzymes Es D1 and Es D2 Homo sapiens
3.1.1.56 0.067
-
 4-Methylumbelliferyl butyrate pH 5.2, 37°C, isoenzymes Es D1 and Es D2 Homo sapiens
3.1.1.56 0.55
-
 4-nitrophenyl butyrate pH 5.2, 37°C, isoenzyme Es D1n Homo sapiens
3.1.1.56 0.83
-
 4-nitrophenyl butyrate pH 5.2, 37°C, isoenzyme Es D2 Homo sapiens
3.1.1.56 0.9
-
 4-nitrophenyl acetate pH 5.2, 37°C, isoenzymes Es D1 and Es D2 Homo sapiens
3.1.1.56 2.5
-
 1-naphthyl acetate pH 5.2, 37°C, isoenzyme Es D1 Homo sapiens
3.1.1.56 2.9
-
 2-naphthyl acetate pH 5.2, 37°C, isoenzyme Es D2 Homo sapiens
3.1.1.56 3.1
-
 1-naphthyl acetate pH 5.2, 37°C, isoenzyme Es D2 Homo sapiens
3.1.1.56 4
-
 2-naphthyl acetate pH 5.2, 37°C, isoenzyme Es D1 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.56 27000
-
 isoenzyme Es D1 and Es D2, gel filtration Homo sapiens
3.1.1.56 34000
-
 1 * 34000, isoenzyme Es D1 and Es D2, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.56 4-methylumbelliferyl acetate + H2O Homo sapiens
-
 4-methylumbelliferone + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.56 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.56 additional information isoenzymes contain very little if any carbohydrate Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.56 isoenzyme Es D1 and Es D2 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.56 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.56 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.56 1-naphthyl acetate + H2O
-
 Homo sapiens 1-naphthol + acetate
-
 ?
3.1.1.56 2-naphthyl acetate + H2O
-
 Homo sapiens 2-naphthol + acetate
-
 ?
3.1.1.56 4-methylumbelliferyl acetate + H2O
-
 Homo sapiens 4-methylumbelliferone + acetate
-
 ?
3.1.1.56 4-methylumbelliferyl butyrate + H2O
-
 Homo sapiens 4-methylumbelliferone + butyrate
-
 ?
3.1.1.56 4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 ?
3.1.1.56 4-nitrophenyl butyrate + H2O
-
 Homo sapiens 4-nitrophenol + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.56 monomer 1 * 34000, isoenzyme Es D1 and Es D2, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.56 8
-
-
 Homo sapiens