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Literature summary extracted from

  • Kim, D.H.; Yang, Y.S.; Jakoby, W.B.
    Aspirin hydrolyzing esterases from rat liver cytosol (1990), Biochem. Pharmacol., 40, 481-487.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.55 bis-4-nitrophenyl phosphate
-
 Rattus norvegicus
3.1.1.55 paraoxon complete inhibition at 0.1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.55 0.00043
-
 1-naphthyl acetate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.00078
-
 1-naphthyl acetate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.0018
-
 phenyl acetate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.0026
-
 phenyl acetate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.0042
-
 4-nitrophenyl acetate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.0053
-
 2-Nitrophenyl acetate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.0059
-
 Acetyl salicylate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.0083
-
 4-nitrophenyl acetate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.0093
-
 4-nitrophenyl butyrate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.0098
-
 2-Nitrophenyl acetate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.017
-
 2-Nitrophenyl butyrate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.019
-
 4-nitrophenyl butyrate pH 7.8, 25°C, isozyme II Rattus norvegicus
3.1.1.55 0.022
-
 2-Nitrophenyl butyrate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.098
-
 4-acetoxybenzoate pH 7.8, 25°C, isozyme I Rattus norvegicus
3.1.1.55 0.11
-
 4-acetoxybenzoate pH 7.8, 25°C, isozyme II Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.55 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.55 additional information
-
 3 other cytoplasmic enzymes active with aspirin are observed differing in their MW of 220000 Rattus norvegicus
3.1.1.55 35000
-
 isozyme I and II, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.55 acetyl salicylate + H2O Rattus norvegicus i.e. aspirin salicylate + acetate
-
 ?
3.1.1.55 acetyl salicylate + H2O Rattus norvegicus active in detoxification salicylate + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.55 Rattus norvegicus
-
 2 isozymes I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.55 isozymes arylesterase I and II, to homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.55 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.55 additional information
-
-
 Rattus norvegicus
3.1.1.55 1.2
-
 purified isozyme I, substrate 4-nitrophenylacetate Rattus norvegicus
3.1.1.55 1.4
-
 purified isozyme II, substrate 4-nitrophenylacetate Rattus norvegicus
3.1.1.55 2.5
-
 purified isozyme I, substrate acetyl salicylate Rattus norvegicus
3.1.1.55 2.55
-
 purified isozyme II, substrate acetyl salicylate Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.1.55 -20°C, pH 6.8, 7% loss of activity per month Rattus norvegicus
3.1.1.55 4°C, pH 6.8, 10% loss of activity per month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.55 1-naphthyl acetate + H2O
-
 Rattus norvegicus 1-naphthol + acetate
-
 ?
3.1.1.55 2-nitrophenyl acetate + H2O
-
 Rattus norvegicus 2-nitrophenol + acetate
-
 ?
3.1.1.55 2-nitrophenyl butyrate + H2O
-
 Rattus norvegicus 2-nitrophenol + butyrate
-
 ?
3.1.1.55 4-acetoxybenzoate + H2O
-
 Rattus norvegicus 4-hydroxybenzoate + acetate
-
 ?
3.1.1.55 4-nitrophenyl acetate + H2O
-
 Rattus norvegicus 4-nitrophenol + acetate
-
 ?
3.1.1.55 4-nitrophenyl butyrate + H2O
-
 Rattus norvegicus 4-nitrophenol + butyrate
-
 ?
3.1.1.55 acetyl salicylate + H2O i.e. aspirin Rattus norvegicus salicylate + acetate
-
 ?
3.1.1.55 acetyl salicylate + H2O active in detoxification Rattus norvegicus salicylate + acetate
-
 ?
3.1.1.55 acetylsalicylate + H2O i.e. aspirin Rattus norvegicus salicylate + acetate
-
 ?
3.1.1.55 phenyl acetate + H2O
-
 Rattus norvegicus phenol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.55 monomer 1 * 35000, both isozymes, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.55 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.55 7.8
-
-
 Rattus norvegicus