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Literature summary extracted from

  • Mack, M.; Bendrat, K.; Zelder, O.; Eckel, E.; Linder, D.; Buckel, W.
    Location of the two genes encoding glutaconate coenzyme A-transferase at the beginning of the hydroxyglutarate operon in Acidaminococcus fermentans (1994), Eur. J. Biochem., 226, 41-51.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.3.12 gctAB genes encoding the two subunits GctA and GctB are cloned, sequenced and overexpressed together in Escherichia coli DH5alpha, the two genes are located at the beginning of the hydroxyglutarate operon, GctA: 320 amino acids protein, GctB: 266 amino acids protein Acidaminococcus fermentans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.3.12 native and recombinant enzyme, expressed in Escherichia coli Acidaminococcus fermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.12 29000
-
 alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE Acidaminococcus fermentans
2.8.3.12 29168
-
 alpha4beta4, 4 * 35725 + 4 * 29168, GctA and GctB, calculated from the DNA sequences Acidaminococcus fermentans
2.8.3.12 35725
-
 alpha4beta4, 4 * 35725 + 4 * 29168, GctA and GctB, calculated from the DNA sequences Acidaminococcus fermentans
2.8.3.12 36000
-
 alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate Acidaminococcus fermentans involved in the conversion of (R)-2-hydroxyglutarate to crotonyl-CoA in the pathway of glutamate fermentation acetate + (R)-2-hydroxyglutaryl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.12 Acidaminococcus fermentans Q59111 and Q59112 5’-part of the hydroxyglutarate operon with gctAB genes encoding Gct, ATCC 25085
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.12 native and recombinant enzyme, expressed in Escherichia coli Acidaminococcus fermentans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.12 additional information
-
-
 Acidaminococcus fermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.12 acetyl-CoA + (E)-glutaconate
-
 Acidaminococcus fermentans acetate + glutaconyl-1-CoA
-
 ?
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate
-
 Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-1-CoA
-
 ?
2.8.3.12 acetyl-CoA + (R)-2-hydroxyglutarate involved in the conversion of (R)-2-hydroxyglutarate to crotonyl-CoA in the pathway of glutamate fermentation Acidaminococcus fermentans acetate + (R)-2-hydroxyglutaryl-CoA
-
 ?
2.8.3.12 glutaryl-CoA + acetate
-
 Acidaminococcus fermentans glutarate + acetyl-CoA
-
 ?
2.8.3.12 additional information residue E-54 of the small subunit GctB is directly involved in catalysis Acidaminococcus fermentans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.12 octamer alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE Acidaminococcus fermentans
2.8.3.12 octamer alpha4beta4, 4 * 35725 + 4 * 29168, GctA and GctB, calculated from the DNA sequences Acidaminococcus fermentans