BRENDA - Enzyme Database show

Site-directed mutagenesis of the substrate-binding cleft of human estrogen sulfotransferase

Hempel, N.; Barnett, A.C.; Bolton-Grob, R.M.; Liyou, N.E.; McManus, M.E.; Biochem. Biophys. Res. Commun. 276, 224-230 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.8.2.4
-
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.8.2.4
H107A
site-directed mutagenesis, humSULT1E1, presence of a histidine residue at position 107 is essential for the production of a functional protein, substitution of this amino acid to alanine results in complete loss of activity towards all substrates tested
Homo sapiens
2.8.2.4
K85A
site-directed mutagenesis, humSULT1E1
Homo sapiens
2.8.2.4
V145E
site-directed mutagenesis, humSULT1E1
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.00005
-
dehydroepiandrosterone
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0001
-
17beta-estradiol
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0001
-
estrone
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0002
-
dehydroepiandrosterone
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.0002
-
estrone
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.0003
-
17beta-estradiol
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.00046
-
3'-phosphoadenylylsulfate
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.00069
-
3'-phosphoadenylylsulfate
pH 7.0, 37°C, mutant SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0036
-
estrone
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
0.0082
-
17beta-estradiol
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
0.01318
-
3'-phosphoadenylylsulfate
pH 7.0, 37°C, mutant SULT1E1-V145E
Homo sapiens
2.8.2.4
0.0607
-
dehydroepiandrosterone
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.0766
-
p-nitrophenol
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.2407
-
dopamine
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
0.6248
-
p-nitrophenol
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.9372
-
p-nitrophenol
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
32.22
-
dopamine
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
79.35
-
dopamine
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
35120
-
calculated from amino acid sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Homo sapiens
-
human
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + dopamine
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + dopamine sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + p-nitrophenol
model substrate, sulfonated by SULT1E1
645902
Homo sapiens
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
-
645902
Homo sapiens
r
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.8.2.4
H107A
site-directed mutagenesis, humSULT1E1, presence of a histidine residue at position 107 is essential for the production of a functional protein, substitution of this amino acid to alanine results in complete loss of activity towards all substrates tested
Homo sapiens
2.8.2.4
K85A
site-directed mutagenesis, humSULT1E1
Homo sapiens
2.8.2.4
V145E
site-directed mutagenesis, humSULT1E1
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.00005
-
dehydroepiandrosterone
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0001
-
17beta-estradiol
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0001
-
estrone
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0002
-
dehydroepiandrosterone
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.0002
-
estrone
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.0003
-
17beta-estradiol
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.00046
-
3'-phosphoadenylylsulfate
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.00069
-
3'-phosphoadenylylsulfate
pH 7.0, 37°C, mutant SULT1E1-K85A
Homo sapiens
2.8.2.4
0.0036
-
estrone
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
0.0082
-
17beta-estradiol
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
0.01318
-
3'-phosphoadenylylsulfate
pH 7.0, 37°C, mutant SULT1E1-V145E
Homo sapiens
2.8.2.4
0.0607
-
dehydroepiandrosterone
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.0766
-
p-nitrophenol
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
0.2407
-
dopamine
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
0.6248
-
p-nitrophenol
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
2.8.2.4
0.9372
-
p-nitrophenol
pH 7.0, 37°C, SULT1E1-V145E
Homo sapiens
2.8.2.4
32.22
-
dopamine
pH 7.0, 37°C, SULT1E1-wild-type
Homo sapiens
2.8.2.4
79.35
-
dopamine
pH 7.0, 37°C, SULT1E1-K85A
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.8.2.4
35120
-
calculated from amino acid sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + dopamine
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + dopamine sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645902
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645902
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + p-nitrophenol
model substrate, sulfonated by SULT1E1
645902
Homo sapiens
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
-
645902
Homo sapiens
r