BRENDA - Enzyme Database show

Effect of Org OD14 (LIVIAL) and its metabolites on human estrogen sulphotransferase activity in the hormone-dependent MCF-7 and T-47D, and the hormone-independent MDA-MB-231, breast cancer cell lines

Chetrite, G.S.; Kloosterboer, H.J.; Philippe, J.C.; Pasqualini, J.R.; Anticancer Res. 19, 269-275 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
17-hydroxy-19-nor-7-methyl-17-pregn-5(10)-en-20-yn-3-one
Org OD14, active substance in Livial (R), 0.0005-0.00005 mM stimulates the formation of estrone by 40-60%, no effect in MDA-MB-231 cells
Homo sapiens
Application
EC Number
Application
Commentary
Organism
2.8.2.4
medicine
formation of inactive estrogen sulfates can cause a reduction in the estrogenic environment which confers a beneficial effect to Org OD14 reducing the risk of breast tumours in long-term hormone replacement treatment
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
OrgOD14
active substance in Livial (r), above 0.05 mM decreases significantly biosynthesis of estrone by 50-60%, no effect in MDA-MB-231 cells
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Homo sapiens
-
human
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
breast cancer cell line
hormone-dependent MCF-7 and T47D, hormone-independent MDA-MB-231
Homo sapiens
-
2.8.2.4
MCF-7 cell
hormone-dependent breast cancer cell line
Homo sapiens
-
2.8.2.4
MDA-MB-231 cell
hormone-independent breast cancer cell line
Homo sapiens
-
2.8.2.4
T47D cell
hormone-dependent breast cancer cell line
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645897
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645897
Homo sapiens
r
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
17-hydroxy-19-nor-7-methyl-17-pregn-5(10)-en-20-yn-3-one
Org OD14, active substance in Livial (R), 0.0005-0.00005 mM stimulates the formation of estrone by 40-60%, no effect in MDA-MB-231 cells
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
2.8.2.4
medicine
formation of inactive estrogen sulfates can cause a reduction in the estrogenic environment which confers a beneficial effect to Org OD14 reducing the risk of breast tumours in long-term hormone replacement treatment
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
OrgOD14
active substance in Livial (r), above 0.05 mM decreases significantly biosynthesis of estrone by 50-60%, no effect in MDA-MB-231 cells
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.8.2.4
cytosol
-
Homo sapiens
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
breast cancer cell line
hormone-dependent MCF-7 and T47D, hormone-independent MDA-MB-231
Homo sapiens
-
2.8.2.4
MCF-7 cell
hormone-dependent breast cancer cell line
Homo sapiens
-
2.8.2.4
MDA-MB-231 cell
hormone-independent breast cancer cell line
Homo sapiens
-
2.8.2.4
T47D cell
hormone-dependent breast cancer cell line
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645897
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645897
Homo sapiens
r