BRENDA - Enzyme Database show

Human liver estrogen sulfotransferase: identification by cDNA cloning and expression

Aksoy, I.A.; Wood, T.C.; Weinshilboum, R.; Biochem. Biophys. Res. Commun. 200, 1621-1629 (1994)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
NaCl
activity of liver EST transiently expressed in COS-1 cells increases 86% in presence of 150 mM
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
2.8.2.4
EST cDNA cloned and expressed in COS-1 cells
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
2,6-Dichloro-4-nitrophenol
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.00017
-
estrone
pH 6.5, 37°C
Homo sapiens
2.8.2.4
0.00085
-
dehydroepiandrosterone
pH 6.5, 37°C
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
Mg2+
1.25 mM increases EST activity 20% with estrone as substrate
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Homo sapiens
-
human
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
645884
Homo sapiens
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
645884
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645884
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645884
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + p-nitrophenol
-
645884
Homo sapiens
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
-
645884
Homo sapiens
r
2.8.2.4
additional information
dopamine is no substrate
645884
Homo sapiens
?
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.8.2.4
30
45
thermal inactivation at 45°C
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.8.2.4
NaCl
activity of liver EST transiently expressed in COS-1 cells increases 86% in presence of 150 mM
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.8.2.4
EST cDNA cloned and expressed in COS-1 cells
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.8.2.4
2,6-Dichloro-4-nitrophenol
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.00017
-
estrone
pH 6.5, 37°C
Homo sapiens
2.8.2.4
0.00085
-
dehydroepiandrosterone
pH 6.5, 37°C
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.8.2.4
Mg2+
1.25 mM increases EST activity 20% with estrone as substrate
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
645884
Homo sapiens
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
645884
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645884
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645884
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + p-nitrophenol
-
645884
Homo sapiens
adenosine 3',5'-bisphosphate + p-nitrophenyl sulfate
-
645884
Homo sapiens
r
2.8.2.4
additional information
dopamine is no substrate
645884
Homo sapiens
?
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.8.2.4
30
45
thermal inactivation at 45°C
Homo sapiens