Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lyon, E.S.; Jakoby, W.B.
    The identity of alcohol sulfotransferases with hydroxysteroid sulfotransferases (1980), Arch. Biochem. Biophys., 202, 474-481.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.2 0.012
-
 dehydroepiandrosterone
-
 Rattus norvegicus
2.8.2.2 0.035
-
 beta-estradiol
-
 Rattus norvegicus
2.8.2.2 0.07
-
 testosterone
-
 Rattus norvegicus
2.8.2.2 0.17
-
 aldosterone pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.29
-
 cortisol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.35
-
 estriol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.44
-
 cortisol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.52
-
 11-deoxycorticosterone pH 7.5, 37°C Rattus norvegicus
2.8.2.2 1
-
 Hexanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 1.2
-
 3-methyl-1-butanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 1.7
-
 1-Pentanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 3
-
 1-butanol
-
 Rattus norvegicus
2.8.2.2 17
-
 2-propanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 24
-
 1-propanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 42
-
 ethanol
-
 Rattus norvegicus
2.8.2.2 47
-
 ethanol pH 7.5, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.2 28000
-
 6 * 28000, SDS-PAGE Rattus norvegicus
2.8.2.2 180000
-
 gel filtration Rattus norvegicus
2.8.2.2 180000
-
 sucrose density gradient centrifugation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone Rattus norvegicus
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Rattus norvegicus
-
 rat
-
2.8.2.2 Rattus norvegicus
-
 Sprague-Dawley, female
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.2 sulfotransferase 1 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.2 0.11
-
 substrate estradiol Rattus norvegicus
2.8.2.2 0.15
-
 substrate n-butanol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylylsulfate + 1-butanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-butanoyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 1-hexanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-hexyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 1-pentanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-pentyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 1-propanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 1-propyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 11-deoxycorticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 11-deoxycorticosterone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 2-propanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 2-propyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + 3-methyl-1-butanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + 3-methyl-1-butyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + aldosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + aldosterone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + beta-estradiol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + beta-estradiol 3-O-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + corticosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + corticosterone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + cortisol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisol sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + cortisone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + cortisone sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + dehydroepiandrosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + estriol poor substrate, relative activity 8% with beta-estradiol as 100% Rattus norvegicus adenosine 3',5'-bisphosphate + estriol sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + ethanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + ethyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + methanol
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + methyl sulfate
-
 r
2.8.2.2 3'-phosphoadenylylsulfate + testosterone
-
 Rattus norvegicus adenosine 3',5'-bisphosphate + testosterone 3-sulfate
-
 r
2.8.2.2 additional information specificity of the enzyme is not limited to hydroxysteroids, a wide range of alcohols serve as sulfate acceptors Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.2 hexamer 6 * 28000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 hydroxysteroid sulfotransferase 1
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.2.2 0.0833
-
 methanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.133
-
 aldosterone pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.15
-
 cortisone pH 7.5, 37°C cosubstrate ethanol Rattus norvegicus
2.8.2.2 0.167
-
 estriol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.183
-
 1-propanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.183
-
 cortisol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.183
-
 11-deoxycorticosterone pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.217
-
 2-propanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.283
-
 1-butanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.417
-
 1-Hexanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.417
-
 3-methyl-1-butanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.45
-
 1-Pentanol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.467
-
 testosterone pH 7.5, 37°C Rattus norvegicus
2.8.2.2 0.583
-
 beta-estradiol pH 7.5, 37°C Rattus norvegicus
2.8.2.2 2
-
 3beta-hydroxyandrost-5-ene-17-one pH 7.5, 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 5.5 6 beta-estradiol and dehydroepiandrosterone as substrate Rattus norvegicus
2.8.2.2 7.5
-
 1-butanol as substrate Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.2.2 Rattus norvegicus preparative isoelectric focusing in sucrose gradients with ampholites in the range pH 3.0-8.0
-
 5