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Literature summary extracted from

  • Hollmann, J.; Niemann, R.; Buddecke, E.
    Purification and characterization of a 3-phosphoadenylylsulfate:chondroitin 6-sulfotransferase from arterial tissue (1986), Biol. Chem. Hoppe-Seyler, 367, 5-13.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.17 Chondroitin sulfates competitive inhibitors Bos taurus
2.8.2.17 lysozyme stimulates 2fold at 1.5 mg/ml Bos taurus
2.8.2.17 poly-L-lysine stimulates 2fold at 1.5 mg/ml Bos taurus
2.8.2.17 protamine stimulates 2fold at 1.5 mg/ml Bos taurus
2.8.2.17 spermidine stimulates 2fold at 1 mM Bos taurus
2.8.2.17 spermine stimulates 2fold at 1 mM Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.17 0.15
-
 chondroitin
-
 Bos taurus
2.8.2.17 0.48
-
 dodecasaccharide derived from chondroitin Bos taurus
2.8.2.17 0.9
-
 decasaccharide derived from chondroitin Bos taurus
2.8.2.17 2.5
-
 Octasaccharide derived from chondroitin Bos taurus
2.8.2.17 5
-
 hexasaccharide derived from chondroitin Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.17 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.17 Ca2+ divalent cation required, Mn2+, Mg2+, Ca2+ Bos taurus
2.8.2.17 Mg2+ divalent cation required, Mn2+, Mg2+, Ca2+ Bos taurus
2.8.2.17 Mn2+ divalent cation required, Mn2+, Mg2+, Ca2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.17 38000
-
 x * 38000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin Bos taurus is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.17 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.17 calf Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.17 artery
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.17 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.17 3'-phosphoadenylyl sulfate + decasaccharide
-
 Bos taurus ?
-
 r
2.8.2.17 3'-phosphoadenylyl sulfate + dodecasaccharide
-
 Bos taurus ?
-
 r
2.8.2.17 3'-phosphoadenylyl sulfate + hexasaccharide
-
 Bos taurus ?
-
 r
2.8.2.17 3'-phosphoadenylyl sulfate + octasaccharide
-
 Bos taurus ?
-
 r
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin specificity towards desulfated chondroitin sulfate and oligosaccharides derived therefrom Bos taurus adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin is believed to act in the course of chondroitin sulfate synthesis in cooperation with, but shortly after the enzymes involved in the chain elongation reaction Bos taurus adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin-derived oligosaccharides even-numbered oligosaccharides with a glucuronic acid at the nonreducing terminus are active as acceptors, their capacity decreasing with decreasing chain length: dodecasaccharide at 64%, decasaccharide at 55%, octasaccharide at 17%, hexasaccharide at 4% of the chondroitin sulfation Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.17 ? x * 38000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.17 6
-
 double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3 Bos taurus
2.8.2.17 7.3
-
 double pH-optimum: 100% at pH 6.0 and 65% at pH 7.3 Bos taurus