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Literature summary extracted from

  • Nakanishi, Y.; Shimizu, M.; Otsu, K.; Kato, S.; Tsuji, M.; Suzuki, S.
    A terminal 6-sulfotransferase catalyzing a synthesis of N-acetylgalactosamine 4,6-bissulfate residue at the nonreducing terminal position of chondroitin sulfate (1981), J. Biol. Chem., 256, 5443-5449.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.17 CaCl2 90% inhibition at 5 mM Coturnix japonica
2.8.2.17 CoCl2 50% inhibition at 5 mM Coturnix japonica
2.8.2.17 MgCl2 40% inhibition at 5 mM Coturnix japonica
2.8.2.17 MnCl2 75% inhibition at 5 mM Coturnix japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.17 0.00011
-
 3'-phosphoadenylylsulfate + UDP-N-acetylgalactosamine 4-sulfate, pH 7, 37°C Coturnix japonica
2.8.2.17 0.00049
-
 chondroitin 4-sulfate pH 7, 37°C Coturnix japonica
2.8.2.17 0.027
-
 UDP-N-acetylgalactosamine 4-sulfate pH 7, 37°C Coturnix japonica
2.8.2.17 0.15
-
 chondroitin 1-phospho-N-acetylgalactosamine 4-sulfate, pH 7, 37°C Coturnix japonica
2.8.2.17 0.36
-
 N-acetylgalactosamine 4-sulfate pH 7, 37°C Coturnix japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin 4-sulfate Coturnix japonica may play a role in synthesis of proteoglycans adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.17 Coturnix japonica
-
 Quail
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.17
-
 Coturnix japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.17 oviduct
-
 Coturnix japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.17 additional information
-
-
 Coturnix japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.17 3'-phosphoadenylylsulfate + 1-phospho-N-acetylgalactosamine 4-sulfate
-
 Coturnix japonica adenosine 3',5'-bisphosphate + 1-phospho-N-acetylgalactosamine 4,6-bissulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin
-
 Coturnix japonica adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin 4-sulfate incorporates sulfate into position 6 of the nonreducing N-acetylgalactosamine 4-sulfate end group of chondroitin, no activity with unsulfated chondroitin Coturnix japonica adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + chondroitin 4-sulfate may play a role in synthesis of proteoglycans Coturnix japonica adenosine 3',5'-bisphosphate + chondroitin 4,6-bissulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + N-acetylgalactosamine 4-sulfate
-
 Coturnix japonica adenosine 3',5'-bisphosphate + N-acetylgalactosamine 4,6-bissulfate
-
 ?
2.8.2.17 3'-phosphoadenylylsulfate + UDP-N-acetylgalactosamine 4-sulfate
-
 Coturnix japonica adenosine 3',5'-bisphosphate + UDP-N-acetylgalactosamine 4,6-bissulfate
-
 ?
2.8.2.17 additional information no substrate: GDPmannose Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: UDP-N-acetylglucosamine 6-sulfate Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: UDPgalactose Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: UDP-N-acetylgalactosamine Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: heparin Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: heparan sulfate Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: UDP-N-acetylglucosamine Coturnix japonica ?
-
 ?
2.8.2.17 additional information no substrate: UDP-N-acetylgalactosamine 6-sulfate Coturnix japonica ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.17 37
-
 assay at Coturnix japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.17 7
-
-
 Coturnix japonica