BRENDA - Enzyme Database show

Steroid sulphotransferase and 17beta-hydroxysteroid dehydrogenase activities in Ishikawa human endometrial adenocarcinoma cells

Chetrite, G.; Pasqualini, J.R.; J. Steroid Biochem. Mol. Biol. 61, 27-34 (1997)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.0000105
-
estradiol
pH 7.4, 37°C, Ishikawa cells
Homo sapiens
2.8.2.15
0.00001
-
17beta-estradiol
pH 7.4, 37°C
Homo sapiens
2.8.2.15
0.0000105
-
estrone
pH 7.4, 37°C
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Homo sapiens
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.8.2.4
Homo sapiens
-
human
-
2.8.2.15
Homo sapiens
-
human
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adenocarcinoma cell
Ishikawa endometrial adenocarcinoma
Homo sapiens
-
2.8.2.15
endometrial cell line
Ishikawa adenocarcinoma
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645734
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estriol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
645734
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645734
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + estriol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estriol sulfate
-
645734
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + estrone
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estrone sulfate
-
645734
Homo sapiens
r
2.8.2.15
additional information
very limited sulfotransferase activity against dehydroepiandrosterone, testosterone and pregnenolone
645734
Homo sapiens
?
-
-
-
-
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.8.2.4
0.0000105
-
estradiol
pH 7.4, 37°C, Ishikawa cells
Homo sapiens
2.8.2.15
0.00001
-
17beta-estradiol
pH 7.4, 37°C
Homo sapiens
2.8.2.15
0.0000105
-
estrone
pH 7.4, 37°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
Homo sapiens
-
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
Homo sapiens
-
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
Homo sapiens
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.8.2.4
adenocarcinoma cell
Ishikawa endometrial adenocarcinoma
Homo sapiens
-
2.8.2.15
endometrial cell line
Ishikawa adenocarcinoma
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.8.2.4
3'-phosphoadenylylsulfate + estradiol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estradiol 3-sulfate
-
645734
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estriol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estriol 3-sulfate
-
645734
Homo sapiens
r
2.8.2.4
3'-phosphoadenylylsulfate + estrone
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estrone 3-sulfate
-
645734
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + 17beta-estradiol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + ?
-
-
-
r
2.8.2.15
3'-phosphoadenylylsulfate + estriol
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estriol sulfate
-
645734
Homo sapiens
r
2.8.2.15
3'-phosphoadenylylsulfate + estrone
-
645734
Homo sapiens
adenosine 3',5'-bisphosphate + estrone sulfate
-
645734
Homo sapiens
r
2.8.2.15
additional information
very limited sulfotransferase activity against dehydroepiandrosterone, testosterone and pregnenolone
645734
Homo sapiens
?
-
-
-
-